PUBLICATION
Bradykinin Receptors in the Zebrafish (Danio rerio)
- Authors
- Bromée, T., Conlon, J.M., and Larhammar, D.
- ID
- ZDB-PUB-050518-6
- Date
- 2005
- Source
- Annals of the New York Academy of Sciences 1040: 246-248 (Journal)
- Registered Authors
- Larhammar, Dan
- Keywords
- bradykinin receptor; inositol phosphates (IP); alanine; d-isomer
- MeSH Terms
-
- Animals
- Cloning, Molecular/methods
- Humans
- Ligands
- Receptors, Bradykinin/chemistry*
- Receptors, Bradykinin/genetics
- Receptors, Bradykinin/metabolism*
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 15891034 Full text @ Ann N Y Acad Sci
Citation
Bromée, T., Conlon, J.M., and Larhammar, D. (2005) Bradykinin Receptors in the Zebrafish (Danio rerio). Annals of the New York Academy of Sciences. 1040:246-248.
Abstract
Ligand interactions of a zebrafish bradykinin (BK) receptor expressed in vitro were characterized by measuring inositol phosphate accumulation. The ligands were analogues of zebrafish BK. Substitutions of Arg1, Gly4, Ser6, Pro7, Leu8, and Arg9 caused greatly reduced potency and maximum response. The Pro3 to Ala analogue had higher potency, but lower maximum response. These and other differences show that the zebrafish BK receptor has a ligand-interaction profile that is distinct from mammalian B1 and B2 receptors and from the previously characterized BK receptor in trout stomach. The results increase our understanding of the evolution of BK receptors and their ligands.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping