PUBLICATION

Shield formation at the onset of zebrafish gastrulation

Authors
Montero, J.A., Carvalho, L., Wilsch-Bräuninger, M., Kilian, B., Mustafa, C., and Heisenberg, C.P.
ID
ZDB-PUB-050211-3
Date
2005
Source
Development (Cambridge, England)   132(6): 1187-1198 (Journal)
Registered Authors
Heisenberg, Carl-Philipp, Kilian, Beate, Montero-Simon, Juan-Antonio
Keywords
E-Cadherin, Shield formation, Cell migration, Gastrulation, Zebrafish
MeSH Terms
  • Animals
  • Body Patterning/physiology
  • Cadherins/physiology
  • Cell Adhesion/physiology
  • Cell Movement/physiology
  • Endoderm/physiology
  • Endoderm/ultrastructure
  • Gastrula/physiology*
  • Gastrula/ultrastructure
  • Mesoderm/physiology
  • Mesoderm/ultrastructure
  • Microscopy, Electron
  • Zebrafish/embryology*
(all 13)
PubMed
15703282 Full text @ Development
Abstract
During vertebrate gastrulation, the three germ layers, ectoderm, mesoderm and endoderm are formed, and the resulting progenitor cells are brought into the positions from which they will later contribute more complex tissues and organs. A core element in this process is the internalization of mesodermal and endodermal progenitors at the onset of gastrulation. Although many of the molecules that induce mesendoderm have been identified, much less is known about the cellular mechanisms underlying mesendodermal cell internalization and germ layer formation. Here we show that at the onset of zebrafish gastrulation, mesendodermal progenitors in dorsal/axial regions of the germ ring internalize by single cell delamination. Once internalized, mesendodermal progenitors upregulate E-Cadherin (Cadherin 1) expression, become increasingly motile and eventually migrate along the overlying epiblast (ectodermal) cell layer towards the animal pole of the gastrula. When E-Cadherin function is compromised, mesendodermal progenitors still internalize, but, with gastrulation proceeding, fail to elongate and efficiently migrate along the epiblast, whereas epiblast cells themselves exhibit reduced radial cell intercalation movements. This indicates that cadherin-mediated cell-cell adhesion is needed within the forming shield for both epiblast cell intercalation, and mesendodermal progenitor cell elongation and migration during zebrafish gastrulation. Our data provide insight into the cellular mechanisms underlying mesendodermal progenitor cell internalization and subsequent migration during zebrafish gastrulation, and the role of cadherin-mediated cell-cell adhesion in these processes.
Genes / Markers
Marker Marker Type Name
cdh1GENEcadherin 1, type 1, E-cadherin (epithelial)
ctnnb1GENEcatenin (cadherin-associated protein), beta 1
foxb1aGENEforkhead box B1a
gata5GENEGATA binding protein 5
gscGENEgoosecoid
tbxtaGENET-box transcription factor Ta
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Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
cdh1MO3-cdh1MRPHLNO
1 - 1 of 1
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Fish
Fish
WT
WT + MO3-cdh1
1 - 2 of 2
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Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available
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Citation
Montero, J.A., Carvalho, L., Wilsch-Bräuninger, M., Kilian, B., Mustafa, C., and Heisenberg, C.P. (2005) Shield formation at the onset of zebrafish gastrulation. Development (Cambridge, England). 132(6):1187-1198.