PUBLICATION
Restricted expression of reggie genes and proteins during early zebrafish development
- Authors
- von Philipsborn, A.C., Ferrer-Vaquer, A., Rivera-Milla, E., Stuermer, C.A., and Málaga-Trillo, E.
- ID
- ZDB-PUB-050209-4
- Date
- 2005
- Source
- The Journal of comparative neurology 482(3): 257-272 (Journal)
- Registered Authors
- Málaga-Trillo, Edward, Rivera-Milla, Eric
- Keywords
- zebrafish neural development, lipid rafts
- MeSH Terms
-
- Animals
- Brain/cytology
- Brain/embryology
- Brain/metabolism
- Cell Membrane/metabolism
- Embryonic Development/genetics
- Embryonic Development/physiology
- Eye/cytology
- Eye/embryology
- Eye/metabolism
- Gene Expression Regulation, Developmental/genetics
- Gene Expression Regulation, Developmental/physiology*
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Mesoderm/cytology
- Mesoderm/metabolism
- Neurons/cytology
- Neurons/metabolism
- Olfactory Mucosa/cytology
- Olfactory Mucosa/embryology
- Olfactory Mucosa/metabolism
- RNA, Messenger/analysis
- Spinal Cord/cytology
- Spinal Cord/embryology
- Spinal Cord/metabolism
- Tissue Distribution
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- PubMed
- 15690489 Full text @ J. Comp. Neurol.
Citation
von Philipsborn, A.C., Ferrer-Vaquer, A., Rivera-Milla, E., Stuermer, C.A., and Málaga-Trillo, E. (2005) Restricted expression of reggie genes and proteins during early zebrafish development. The Journal of comparative neurology. 482(3):257-272.
Abstract
Reggies are plasma membrane-associated proteins and characteristic markers of lipid-raft microdomains. They are highly conserved from flies to humans and have been implicated in axon regeneration and cell process and contact formation, possibly providing functional platforms for cell-signaling in neurons and other cell types. We analyzed reggie mRNA and protein expression patterns during early zebrafish development. All three zebrafish genes, re-1a, -2a, and -2b, span a considerably diverse set of expression patterns, and their proteins are induced maternally, showing ubiquitous expression at early stages. Although re-2a mRNA can be observed in differentiating neurons in the brain, spinal cord, and neurogenic placodes, re-2b is transcribed mainly in head mesoderm, in neural crest derivates, and along somite boundaries. re-1a mRNA is present at high levels in expression domains that overlap with the combined expression pattern of both re-2 genes except at the somites, where it complements the pattern of re-2b. Immunostaining on embryos reveals reggie protein localization at the cell membrane, at cell-cell contacts, and along all early axon tracts. The early phase of reggie expression suggests a basic and ubiquitous function during the first stages of embryogenesis and into the gastrula period. Upon segmentation, a second phase of expression shows distinctly localized expression patterns, indicating tissue-specific roles and an involvement of re-1a/re-2a in neural development. 2004 Wiley-Liss, Inc.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping