PUBLICATION
Molecular cloning and functional characterization of a high-affinity zinc importer (DrZIP1) from zebrafish (Danio rerio)
- Authors
- Qiu, A., Shayeghi, M., and Hogstrand, C.
- ID
- ZDB-PUB-050203-1
- Date
- 2005
- Source
- The Biochemical journal 388(Pt 3): 745-54 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cation Transport Proteins/chemistry
- Cation Transport Proteins/genetics*
- Cation Transport Proteins/metabolism*
- Cell Line
- Cloning, Molecular
- Conserved Sequence
- Gene Expression Profiling
- Humans
- Molecular Sequence Data
- Oocytes/metabolism
- Phylogeny
- Protein Transport
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Salmon
- Sequence Alignment
- Sequence Homology, Amino Acid
- Tetraodontiformes
- Xenopus laevis
- Zebrafish/genetics*
- Zinc/metabolism*
- PubMed
- 15683366 Full text @ Biochem. J.
Citation
Qiu, A., Shayeghi, M., and Hogstrand, C. (2005) Molecular cloning and functional characterization of a high-affinity zinc importer (DrZIP1) from zebrafish (Danio rerio). The Biochemical journal. 388(Pt 3):745-54.
Abstract
Zinc is a vital micronutrient to all organisms and a potential toxicant to aquatic animals. It is therefore of importance to understand the mechanism of zinc regulation. In the present study, we molecularly cloned and functionally characterized a zinc transporter of the SLC39A family (commonly referred to as the ZIP family) from the gill of zebrafish, Danio rerio (DrZIP1). DrZIP1 protein was found to localize at the plasma membrane and to function as a zinc uptake transporter when being expressed in either chinook salmon (Oncorhynchus tshawytscha) embryonic cells (CHSE214) cells or Xenopus laevis oocytes. In comparison with pufferfish transporter proteins (FrZIP2 and FrECaC) that have shown to facilitate cellular zinc uptake, DrZIP1 appears to have high affinity to bind and transport zinc, suggesting that it may be a high affinity zinc uptake transporter (Km<0.5muM)) in fish. Orthologs of DrZIP1 were also identified in both freshwater and seawater pufferfish (Tetraodon nigroviridis and Takifugu rubripes), indicating that these proteins may be functionally conserved among different fish species. DrZIP1 mRNA is expressed in all the tissues examined in this study, and thus DrZIP1 may be a constitutive zinc uptake transporter in many cell types of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping