PUBLICATION

Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish

Authors
Rhodes, J., Hagen, A., Hsu, K., Deng, M., Liu, T.X., Look, A.T., and Kanki, J.P.
ID
ZDB-PUB-050104-3
Date
2005
Source
Developmental Cell   8(1): 97-108 (Journal)
Registered Authors
Hsu, Karl, Kanki, John, Liu, Ting Xi, Look, A. Thomas, Rhodes, Jennifer
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/physiology
  • Cell Movement/physiology
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology*
  • Embryonic Induction
  • Erythroid Precursor Cells/physiology*
  • Erythroid-Specific DNA-Binding Factors
  • Flow Cytometry/methods
  • GATA1 Transcription Factor
  • Gene Expression Regulation, Developmental/physiology
  • Genotype
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Hematopoiesis/physiology
  • In Situ Hybridization/methods
  • Microinjections/methods
  • Models, Biological
  • Myeloid Progenitor Cells/physiology*
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology*
  • RNA, Messenger/biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Trans-Activators/genetics
  • Trans-Activators/physiology*
  • Transcription Factors/genetics
  • Transcription Factors/physiology*
  • Transplantation/methods
  • Zebrafish/embryology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
(all 32)
PubMed
15621533 Full text @ Dev. Cell
Abstract
The zebrafish is a powerful model system for investigating embryonic vertebrate hematopoiesis, allowing for the critical in vivo analysis of cell lineage determination. In this study, we identify zebrafish myeloerythroid progenitor cells (MPCs) that are likely to represent the functional equivalent of mammalian common myeloid progenitors. Utilizing transgenic pu.1-GFP fish, real-time MPC differentiation was correlated with dynamic changes in cell motility, morphology, and gene expression. Unlike mammalian hematopoiesis, embryonic zebrafish myelopoiesis and erythropoiesis occur in anatomically separate locations. Gene knockdown experiments and transplantation assays demonstrated the reciprocal negative regulation of pu.1 and gata1 and their non-cell-autonomous regulation that determines myeloid versus erythroid MPC fate in the distinct blood-forming regions. Furthermore, forced expression of pu.1 in the bloodless mutant cloche resulted in myelopoietic rescue, providing intriguing evidence that this gene can function in the absence of some stem cell genes, such as scl, in governing myelopoiesis.
Genes / Markers
Marker Marker Type Name
egr2bGENEearly growth response 2b
gata1aGENEGATA binding protein 1a
hbae1.1GENEhemoglobin, alpha embryonic 1.1
lcp1GENElymphocyte cytosolic protein 1 (L-plastin)
lmo2GENELIM domain only 2 (rhombotin-like 1)
lyzGENElysozyme
mpxGENEmyeloid-specific peroxidase
myod1GENEmyogenic differentiation 1
nkx2.5GENENK2 homeobox 5
runx1GENERUNX family transcription factor 1
1 - 10 of 13
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Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    m39
      		Deficiency
      m651
        		Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        gata1aMO1-gata1aMRPHLNO
        spi1bMO1-spi1bMRPHLNO
        1 - 2 of 2
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        Fish
        Fish
        AB
        AB + MO1-gata1a
        AB + MO1-spi1b
        Df(Chr13:klhdc3,lclat1,npas4l,slc30a10)m39 (INDO)
        gata1am651/m651 (AB)
        tbx16b104/b104 (AB)
        tbx16b104/b104 + MO1-spi1b (AB)
        1 - 7 of 7
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        Antibodies
        No data available
        Orthology
        Engineered Foreign Genes
        No data available
        Mapping
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        Citation
        Rhodes, J., Hagen, A., Hsu, K., Deng, M., Liu, T.X., Look, A.T., and Kanki, J.P. (2005) Interplay of pu.1 and gata1 determines myelo-erythroid progenitor cell fate in zebrafish. Developmental Cell. 8(1):97-108.