PUBLICATION
Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish
- Authors
- Söllner, C., Schwarz, H., Geisler, R., and Nicolson, T.
- ID
- ZDB-PUB-041014-4
- Date
- 2004
- Source
- Development genes and evolution 214(12): 582-590 (Journal)
- Registered Authors
- Geisler, Robert, Nicolson, Teresa, Söllner, Christian
- Keywords
- Otolith biogenesis, Otopetrin 1, backstroke, Starmaker, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cloning, Molecular
- Ear, Inner/cytology
- Epithelium/chemistry
- Epithelium/metabolism
- Gene Expression/drug effects
- Membrane Proteins/genetics
- Membrane Proteins/physiology*
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides, Antisense/genetics
- Oligodeoxyribonucleotides, Antisense/pharmacology
- Otolithic Membrane/embryology*
- Otolithic Membrane/metabolism
- Protein Transport/genetics
- Protein Transport/physiology
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology
- Zebrafish Proteins/analysis*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 15480759 Full text @ Dev. Genes Evol.
Citation
Söllner, C., Schwarz, H., Geisler, R., and Nicolson, T. (2004) Mutated otopetrin 1 affects the genesis of otoliths and the localization of Starmaker in zebrafish. Development genes and evolution. 214(12):582-590.
Abstract
Otoliths in bony fishes and otoconia in mammals are composite crystals consisting of calcium carbonate and proteins. These biominerals are part of the gravity and linear acceleration detection system of the inner ear. Mutations in otopetrin 1 have been shown to result in lack of otoconia in tilted and mergulhador mutant mice. The molecular function of Otopetrin 1, a novel protein that contains ten predicted transmembrane domains, however, has remained elusive. Here we show that a mutation in the orthologous gene in zebrafish is responsible for the complete absence of otoliths in backstroke mutants. We examined the localization of Starmaker, a secreted protein that is highly abundant in otoliths in backstroke mutants. Starmaker protein accumulated within cells of the otic epithelium, indicating a possible defect in secretion. Our data suggest that Otopetrin 1 in zebrafish may be involved in the protein trafficking of components required for formation of biominerals in the ear.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping