PUBLICATION
Evolution of sialic acid-binding proteins: molecular cloning and expression of fish Siglec-4
- Authors
- Lehmann, F., Gathje, H., Kelm, S., and Dietz, F.
- ID
- ZDB-PUB-040706-4
- Date
- 2004
- Source
- Glycobiology 14(11): 959-968 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Alternative Splicing/genetics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Adhesion Molecules/chemistry
- Cell Adhesion Molecules/genetics
- Cell Adhesion Molecules/metabolism
- Cloning, Molecular
- Evolution, Molecular*
- Exons/genetics
- Fish Proteins/chemistry
- Fish Proteins/genetics*
- Fish Proteins/metabolism*
- Gene Expression Profiling*
- Genomics
- Humans
- Introns/genetics
- Lectins/chemistry
- Lectins/genetics*
- Lectins/metabolism*
- Molecular Sequence Data
- N-Acetylneuraminic Acid/metabolism*
- Protein Binding
- Sialic Acid Binding Immunoglobulin-like Lectins
- Substrate Specificity
- Takifugu/genetics*
- PubMed
- 15229193 Full text @ Glycobiology
Citation
Lehmann, F., Gathje, H., Kelm, S., and Dietz, F. (2004) Evolution of sialic acid-binding proteins: molecular cloning and expression of fish Siglec-4. Glycobiology. 14(11):959-968.
Abstract
Siglecs are the largest family of sialic-acid recognizing lectins identified so far with eleven members in the human genome. Most of these siglecs are exclusively expressed by cells of the immune system. Comparison of different mammalian species has revealed differential and complex evolutionary paths for this protein family even within the primate lineage. To understand the evolution of siglecs, in particular the origin of this family, we investigated the occurrence of corresponding genes in bony fish. Interestingly, only unambiguous orthologues of mammalian Siglec-4, a cell adhesion molecule expressed exclusively in the nervous system, could be identified in the genomes of fugu and zebrafish, whereas no obvious orthologues of the other mammalian siglecs were found. As in mammals fish Siglec-4 expression is restricted to nervous tissues as demonstrated by Northern blot. Expressed as recombinant protein, fish Siglec-4 binds to sialic acids with a specificity similar to the mammalian orthologues. Relatively low sequence similarities in the cytoplasmic tail as well as an additional splice variant found in fish Siglec-4 suggest alternative signaling pathways compared to mammalian species. Our observations suggest that this siglec occurs at least in the nervous system of all vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping