PUBLICATION
Zebrafish reveals different and conserved features of vertebrate neuroglobin gene structure, expression pattern and ligand binding
- Authors
- Fuchs, C., Heib, V., Kiger, L., Haberkamp, M., Roesner, A., Schmidt, M., Hamdane, D., Marden, M.C., Hankeln, T., and Burmester, T.
- ID
- ZDB-PUB-040514-1
- Date
- 2004
- Source
- The Journal of biological chemistry 279(23): 24116-24122 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Binding, Competitive
- Blotting, Western
- Chlorides/chemistry
- Cloning, Molecular
- Conserved Sequence
- DNA, Complementary/metabolism
- Diffusion
- Exons
- Gene Expression Regulation
- Gills/metabolism
- Globins/genetics*
- Histidine/chemistry
- In Situ Hybridization
- Introns
- Kinetics
- Ligands
- Microscopy, Fluorescence
- Mitochondria/metabolism
- Models, Genetic
- Nerve Tissue Proteins/genetics*
- Olfactory Pathways/metabolism
- Oxygen/metabolism
- RNA, Messenger/metabolism
- Recombinant Proteins/chemistry
- Retina/metabolism
- Spectrophotometry
- Zebrafish
- PubMed
- 15140880 Full text @ J. Biol. Chem.
Citation
Fuchs, C., Heib, V., Kiger, L., Haberkamp, M., Roesner, A., Schmidt, M., Hamdane, D., Marden, M.C., Hankeln, T., and Burmester, T. (2004) Zebrafish reveals different and conserved features of vertebrate neuroglobin gene structure, expression pattern and ligand binding. The Journal of biological chemistry. 279(23):24116-24122.
Abstract
Neuroglobin has been identified as a respiratory protein that is primarily expressed in the mammalian nervous system. Here we present the first detailed analysis of neuroglobin from a non-mammalian vertebrate, the zebrafish Danio rerio. The zebrafish neuroglobin gene reveals a mammalian-type exon-intron pattern in the coding region (B12.2, E11.0, G7.0), plus an additional 5' non-coding exon. Like the mammalian neuroglobin, the zebrafish protein displays a hexacoordinate deoxy-binding scheme. Flash photolysis kinetics show the competitive binding on the ms timescale of external ligands and the distal histidine, resulting in an oxygen affinity of 1 torr. Western blotting, immune staining and mRNA in situ hybridization demonstrate neuroglobin expression in the fish central nervous system, the retina, but also in the gills. Neurons containing neuroglobin have a widespread distribution in the brain, but are also present in the olfactory system. In the fish retina, neuroglobin is mainly present in the inner segments of the photoreceptor cells. In the gills, the chloride cells were identified to express neuroglobin. Neuroglobin appears to be associated with mitochondria-rich cell types and thus oxygen consumption rates, suggesting a myoglobin-like function of this protein in facilitated oxygen diffusion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping