PUBLICATION
Molecular cloning and characterization of thyroid hormone receptors in teleost fish
- Authors
- Marchand, O., Safi, R., Escriva, H., van Rompaey, E., Prunet, P., and Laudet, V.
- ID
- ZDB-PUB-040205-4
- Date
- 2001
- Source
- Journal of molecular endocrinology 26(1): 51-65 (Journal)
- Registered Authors
- Escriva, Hector, Laudet, Vincent, Marchand, Oriane, Safi, Rachid
- Keywords
- none
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
- Fishes
- Molecular Sequence Data
- Phylogeny
- Protein Isoforms/genetics
- Receptors, Thyroid Hormone/genetics*
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- PubMed
- 11174854 Full text @ J. Mol. Endocrinol.
Citation
Marchand, O., Safi, R., Escriva, H., van Rompaey, E., Prunet, P., and Laudet, V. (2001) Molecular cloning and characterization of thyroid hormone receptors in teleost fish. Journal of molecular endocrinology. 26(1):51-65.
Abstract
Thyroid hormones are pleiotropic factors important for many developmental and physiological functions in vertebrates. Their effects are mediated by two specific receptors (TRalpha and TRbeta) which are members of the nuclear hormone receptor superfamily. To clarify the function of these receptors, our laboratory has started a comparative study of their role in teleost fish. This type of approach has been hampered by the isolation of specific clones for each fish species studied. In this report, we describe an efficient reverse transcription/PCR procedure that allows the isolation of large fragments corresponding to TRalpha and TRbeta of a wide range of teleost fish. Phylogenetic analysis of these receptors revealed a placement consistent with their origin, sequences from teleost fish being clearly monophyletic for both TRalpha and TRbeta. Interestingly, this approach allowed us to isolate (from tilapia and salmon) several new TRalpha or TRbeta isoforms resulting from alternative splicing. These isoforms correspond to expressed transcripts and thus may have an important physiological function. In addition, we isolated a cDNA encoding TRbeta in the Atlantic salmon (Salmo salar) encoding a functional thyroid hormone receptor which binds specific thyroid hormone response elements and regulates transcription in response to thyroid hormones.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping