PUBLICATION
Heat- and cold-inducible regulation of HSP70 expression in zebrafish ZF4 cells
- Authors
- Airaksinen, S., Jokilehto, T., Rabergh, C.M., and Nikinmaa, M.
- ID
- ZDB-PUB-031009-3
- Date
- 2003
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 136(2): 275-282 (Journal)
- Registered Authors
- Nikinmaa, Mikko
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Line
- Cold Temperature*
- Gene Expression Regulation*
- HSP70 Heat-Shock Proteins/genetics*
- Heat-Shock Response/genetics*
- Hot Temperature*
- Protein Isoforms/genetics
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Time Factors
- Transcription Factors/genetics
- Zebrafish/genetics*
- Zebrafish Proteins/genetics
- PubMed
- 14529753 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Airaksinen, S., Jokilehto, T., Rabergh, C.M., and Nikinmaa, M. (2003) Heat- and cold-inducible regulation of HSP70 expression in zebrafish ZF4 cells. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 136(2):275-282.
Abstract
Elevated temperature induces a rapid heat shock transcription factor (HSFs)-mediated expression of heat shock (hsp) genes. The effect of cold exposure on hsp gene expression has hardly been investigated, although ectothermic animals experience both cold and heat stress. We have previously shown in zebrafish that the expression of hsf1a and a unique isoform hsf1b vary in a tissue-specific manner upon heat stress. In the current study, using a zebrafish (Danio rerio) embryonic cell line (ZF4), we have compared the effects of heat shock (28-->37 degrees C) vs. cold shock (28-->20 degrees C) on the expression of ahsf1a, zhsf1b and hsp70. Concomitantly, the suitability of the ZF4 cells as a model system was verified. The expression pattern of HSP70 proteins following heat or cold exposure is distinct, and the total HSP70 level is upregulated or stable, respectively. Moreover, heat exposure specifically increases the ratio of zhsf1a/b expression (10-fold), whereas cold exposure decreases it to one half. These data suggest that the zhsf1a/zhsf1b ratio is regulated in a temperature-dependent manner, and the ratio may be indicative of the stressor-specific HSP70 expression. Furthermore, the response in ZF4 cells upon heat shock resembles the response observed in zebrafish liver and thus, supports the use of this cell line in stress response studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping