PUBLICATION
Requirement for intracellular calcium modulation in zebrafish dorsal-ventral patterning
- Authors
- Westfall, T.A., Hjertos, B., and Slusarski, D.C.
- ID
- ZDB-PUB-030728-8
- Date
- 2003
- Source
- Developmental Biology 259(2): 380-391 (Journal)
- Registered Authors
- Hjertos, Beth Ellen, Slusarski, Diane C.
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning/genetics*
- Calcium/metabolism*
- Cytoskeletal Proteins/drug effects
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism
- Diphosphonates/pharmacology
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian
- Enzyme Inhibitors/pharmacology
- Estrenes/pharmacology
- Gene Expression Regulation, Developmental
- Models, Biological
- Phenotype
- Phosphatidylinositols/metabolism
- Pyrrolidinones/pharmacology
- Signal Transduction
- Thapsigargin/pharmacology
- Trans-Activators/drug effects
- Type C Phospholipases/antagonists & inhibitors
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins
- beta Catenin
- PubMed
- 12871708 Full text @ Dev. Biol.
Citation
Westfall, T.A., Hjertos, B., and Slusarski, D.C. (2003) Requirement for intracellular calcium modulation in zebrafish dorsal-ventral patterning. Developmental Biology. 259(2):380-391.
Abstract
The phosphoinositide (PI) cycle is an important signal transduction pathway that, upon activation, generates intracellular second messengers and leads to calcium release. To determine whether PI cycle-mediated intracellular calcium release is required for body plan formation, we systematically dissect PI cycle function in the zebrafish (Danio rerio ). We inhibit PI cycle function at three different steps and deplete internal calcium stores, demonstrating an impact on endogenous calcium release and Wnt/beta-catenin signaling. Inhibition of endogenous calcium modulation induces hyperdorsalized phenotypes in a dose-dependent manner. Ectopic dorsal-signaling centers are generated in PI cycle- inhibited embryos as demonstrated by altered beta-catenin subcellular localization and ectopic expression of beta-catenin target genes. These results provide evidence that modulation of calcium release is critical for early embryonic patterning and acts by influencing the stabilization of beta-catenin protein.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping