PUBLICATION
Teleost multiple tissue (tmt) opsin: a candidate photopigment regulating the peripheral clocks of zebrafish?
- Authors
- Moutsaki, P., Whitmore, D., Bellingham, J., Sakamoto, K., David-Gray, Z.K., and Foster, R.G.
- ID
- ZDB-PUB-030408-8
- Date
- 2003
- Source
- Brain research. Molecular brain research 112(1-2): 135-145 (Journal)
- Registered Authors
- Whitmore, David
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence/genetics
- Animals
- Base Sequence/genetics
- Biological Clocks/physiology*
- Brain/metabolism
- Cell Line/cytology
- Cell Line/metabolism*
- DNA, Complementary/analysis
- DNA, Complementary/genetics
- Evolution, Molecular
- Gene Expression Regulation/genetics*
- Genomic Library
- Molecular Sequence Data
- Photoperiod*
- Phylogeny
- Rod Opsins/genetics
- Rod Opsins/isolation & purification*
- Sequence Homology, Amino Acid
- Takifugu/genetics
- Takifugu/metabolism*
- Viscera/metabolism
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 12670711 Full text @ Brain Res. Mol. Brain Res.
Citation
Moutsaki, P., Whitmore, D., Bellingham, J., Sakamoto, K., David-Gray, Z.K., and Foster, R.G. (2003) Teleost multiple tissue (tmt) opsin: a candidate photopigment regulating the peripheral clocks of zebrafish?. Brain research. Molecular brain research. 112(1-2):135-145.
Abstract
Isolated organs and cell lines from zebrafish exhibit circadian oscillations in clock gene expression that can be entrained to a 24-h light/dark cycle. The mechanism underlying this cellular photosensitivity is unknown. We report the identification of a novel opsin family, tmt-opsin, that has a genomic structure characteristic of vertebrate photopigments, an amino acid identity equivalent to the known photopigment opsins, and the essential residues required for photopigment function. Significantly, tmt-opsin is expressed in a wide variety of neural and non-neural tissues, including a zebrafish embryonic cell line that exhibits a light entrainable clock. Collectively the data suggest that tmt-opsin is a strong candidate for the photic regulation of zebrafish peripheral clocks.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping