PUBLICATION

N-Cadherin is essential for retinal lamination in the zebrafish

Authors

Erdmann, B., Kirsch, F.P., Rathjen, F.G., and Moré, M.I.

ID

ZDB-PUB-030307-7

Date

2003

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 226(3): 570-577 (Journal)

Registered Authors

Keywords

none

MeSH Terms

Animals
Cadherins/genetics*
Cadherins/metabolism*
Epithelial Cells/physiology
Gene Expression Regulation, Developmental*
Lens, Crystalline/cytology
Lens, Crystalline/embryology
Microscopy, Electron
Mutation
Photoreceptor Cells, Vertebrate/physiology
Photoreceptor Cells, Vertebrate/ultrastructure
Retina/embryology*
Retina/physiology*
Synapses/physiology
Zebrafish

(all 15)

PubMed

12619142 Full text @ Dev. Dyn.

Abstract

N-Cadherin is one of the major Ca(2+)-dependent cell adhesion proteins in the developing nervous system. Here, we analyze eye development in the zebrafish N-cadherin loss-of-function mutant parachute(paR2.10) (pac(paR2.10)). The zebrafish visual system is fully developed by the time pac(paR2.10) mutants show lethality at day 5. Already at 24 hr postfertilization (hpf), mutant retinal cells are more disorganized and more rounded than in wild-type. At later stages, mutant retinae display a severe lamination defect with rosette formation (mostly islands of plexiform layer tissue surrounded by inner nuclear layer or photoreceptor cells), even though all major classes of cell types appear to be present as determined by histology. Of interest, electron microscopy reveals that the islands of plexiform layer tissue contain a normal amount of synapses with normal morphology. Although mutant photoreceptor cells are sometimes deformed, all typical structural components are present, including the membranous discs for rhodopsin storage. The lens fibers of the pac(paR2.10) mutants develop completely normally, but in some cases, lens epithelial cells round up and become multilayered. We conclude that cell adhesion mediated by N-cadherin is of major importance for retinal lamination and involved in maintenance of the lens epithelial sheet, but is not essential for the formation of photoreceptor ultrastructure or for synaptogenesis.

Genes / Markers

Marker	Marker Type	Name
cdh2	GENE	cadherin 2, type 1, N-cadherin (neuronal)

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
cdh2^r210/r210	standard conditions	Prim-5	retina disorganized, abnormal	Fig. 1
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	lens disoriented, abnormal	Fig. 1
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	maintenance of epithelial cell apical/basal polarity disrupted, abnormal	Fig. 1
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	maintenance of epithelial cell apical/basal polarity disrupted, abnormal	Fig. 2
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	retinal ganglion cell layer mislocalised, abnormal	Fig. 1
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	retinal ganglion cell layer mislocalised, abnormal	Fig. 2
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	retinal inner plexiform layer structure, abnormal	Fig. 1
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	retinal inner plexiform layer structure, abnormal	Fig. 2
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	retinal outer plexiform layer structure, abnormal	Fig. 1
cdh2^r210/r210	standard conditions	Prim-5 to Protruding-mouth	retinal outer plexiform layer structure, abnormal	Fig. 2

1 - 10 of 20

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
r210		Point Mutation	cdh2

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
cdh2^r210/r210

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Erdmann et al., 2003 ZDB-PUB-030307-7 PMID:12619142

N-Cadherin is essential for retinal lamination in the zebrafish

Erdmann et al., 2003

ZDB-PUB-030307-7
PMID:12619142