PUBLICATION

Maternal induction of ventral fate by zebrafish radar

Authors
Sidi, S., Goutel, C., Peyriéras, N., and Rosa, F.M.
ID
ZDB-PUB-030304-6
Date
2003
Source
Proceedings of the National Academy of Sciences of the United States of America   100(6): 3315-3320 (Journal)
Registered Authors
Goutel, Carole, Rosa, Frederic, Sidi, Samuel
Keywords
none
MeSH Terms
  • Animals
  • Base Sequence
  • Body Patterning
  • Bone Morphogenetic Proteins/genetics*
  • Bone Morphogenetic Proteins/physiology*
  • Embryonic Induction
  • Female
  • Gene Expression Regulation, Developmental
  • Growth Differentiation Factor 6
  • In Situ Hybridization
  • Mutation
  • Phenotype
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins*
(all 17)
PubMed
12601179 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
In vertebrate embryos, maternal determinants are thought to preestablish the dorsoventral axis by locally activating zygotic ventral- and dorsal-specifying genes, e.g., genes encoding bone morphogenetic proteins (BMPs) and BMP inhibitors, respectively. Whereas the canonical Wnt/beta-catenin pathway fulfills this role dorsally, the existence of a reciprocal maternal ventralizing signal remains hypothetical. Maternal noncanonical Wnt/Ca(2+) signaling may promote ventral fates by suppressing Wnt/beta-catenin dorsalizing signals; however, whether any maternal determinant is directly required for the activation of zygotic ventral-specifying genes is unknown. Here, we show that such a function is achieved, in part, in the zebrafish embryo by the maternally encoded transforming growth factor beta (TGF-beta) signaling molecule, Radar. Loss-of-function experiments, together with epistasis analyses, identify maternal Radar as an upstream activator of bmps expression. Maternal induction of bmps by Radar is essential for zebrafish development as its removal results in larval-lethal dorsalized phenotypes. Double-morphant analyses further suggest that Radar functions through the TGF-beta receptor Alk8 to initiate the expression of bmp genes. Our results support the existence of a previously uncharacterized maternal ventralizing pathway. They might further indicate that maternal TGF-beta/Rdr and Wnt/Ca(2+) pathways complementarily specify ventral cell fates, with the former triggering bmps expression and the latter indirectly repressing genes encoding BMP antagonists.
Genes / Markers
Marker Marker Type Name
acvr1lGENEactivin A receptor, type 1 like
bmp2bGENEbone morphogenetic protein 2b
bmp4GENEbone morphogenetic protein 4
bmp7aGENEbone morphogenetic protein 7a
chrdGENEchordin
gdf6aGENEgrowth differentiation factor 6a
ndr1GENEnodal-related 1
ndr2GENEnodal-related 2
smad5GENESMAD family member 5
ventGENEventral expressed homeobox
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Figures
Figure Gallery (4 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
bmp2bWTstandard conditionsSphereISH
bmp2bWTstandard conditions50%-epibolyISH
bmp2bWTstandard conditionsSphereISH
bmp2bWT + MO2-gdf6astandard conditionsSphereISH
bmp4WTstandard conditionsSphereISH
bmp4WTstandard conditions50%-epibolyISH
bmp4WTstandard conditionsSphereISH
bmp4WT + MO2-gdf6astandard conditionsSphereISH
bmp7aWTstandard conditionsSphereISH
bmp7aWTstandard conditions50%-epibolyISH
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
dtc24
    		Point Mutation
    ta72a
      		Point Mutation
      tt250
        		Point Mutation
        ty68a
          		Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          gdf6aMO1-gdf6aMRPHLNO
          gdf6aMO2-gdf6aMRPHLNO
          1 - 2 of 2
          Show
          Fish
          Fish
          bmp2bta72a/ta72a (TU)
          bmp7aty68a/ty68a
          chrdtt250/tt250
          smad5dtc24/dtc24 (TU)
          WT
          WT + MO2-gdf6a
          1 - 6 of 6
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          Antibodies
          Orthology
          Engineered Foreign Genes
          No data available
          Mapping
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          Citation
          Sidi, S., Goutel, C., Peyriéras, N., and Rosa, F.M. (2003) Maternal induction of ventral fate by zebrafish radar. Proceedings of the National Academy of Sciences of the United States of America. 100(6):3315-3320.