PUBLICATION
Phylogenetic comparison of the pre-mRNA adenosine deaminase ADAR2 genes and transcripts: conservation and diversity in editing site sequence and alternative splicing patterns
- Authors
- Slavov, D. and Gardiner, K.
- ID
- ZDB-PUB-030117-6
- Date
- 2002
- Source
- Gene 299(1-2): 83-94 (Journal)
- Registered Authors
- Keywords
- RNA editing; Inosine; Glutamate receptor; Alternative splicing; Genomic organization
- MeSH Terms
-
- Adenosine Deaminase/genetics*
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Chickens
- Conserved Sequence/genetics
- DNA/chemistry
- DNA/genetics
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Exons/genetics
- Genes/genetics
- Humans
- Introns/genetics
- Mice
- Molecular Sequence Data
- Nucleic Acid Conformation
- Phylogeny*
- RNA Precursors/genetics*
- RNA-Binding Proteins
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Takifugu
- Transcription, Genetic
- Zebrafish
- PubMed
- 12459255 Full text @ Gene
Citation
Slavov, D. and Gardiner, K. (2002) Phylogenetic comparison of the pre-mRNA adenosine deaminase ADAR2 genes and transcripts: conservation and diversity in editing site sequence and alternative splicing patterns. Gene. 299(1-2):83-94.
Abstract
denosine deaminase that acts on RNA -2 (ADAR2) is a member of a family of vertebrate genes that encode adenosine (A)-to-inosine (I) RNA deaminases, enzymes that deaminate specific A residues in specific pre-mRNAs to produce I. Known substrates of ADAR2 include sites within the coding regions of pre-mRNAs of the ionotropic glutamate receptors, GluR2-6, and the serotonin receptor, 5HT2C. Mammalian ADAR2 expression is itself regulated by A-to-I editing and by several alternative splicing events. Because the biological consequences of ADAR2 function are significant, we have undertaken a phylogenetic comparison of these features. Here we report a comparison of cDNA sequences, genomic organization, editing site sequences and patterns of alternative splicing of ADAR2 genes from human, mouse, chicken, pufferfish and zebrafish. Coding sequences and intron/exon organization are highly conserved. All ADAR2 genes show evidence of transcript editing with required sequences and predicted secondary structures very highly conserved. Patterns and levels of editing and alternative splicing vary among organisms, and include novel N-terminal exons and splicing events.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping