PUBLICATION

Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling

Authors
Houart, C., Caneparo, L., Heisenberg, C.P., Barth, K.A., Take-uchi, M., and Wilson, S.W.
ID
ZDB-PUB-021017-71
Date
2002
Source
Neuron   35(2): 255-265 (Journal)
Registered Authors
Barth, Anukampa, Caneparo, Luca, Heisenberg, Carl-Philipp, Houart, Corinne, Take-uchi, Masaya, Wilson, Steve
Keywords
none
MeSH Terms
  • Animals
  • Body Patterning/genetics*
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins/genetics
  • Bone Morphogenetic Proteins/metabolism
  • Cell Differentiation/genetics
  • Cell Lineage/genetics
  • Cytoskeletal Proteins
  • Denervation
  • Gastrula/cytology
  • Gastrula/metabolism*
  • Gene Expression Regulation/physiology*
  • Immunohistochemistry
  • Mesencephalon/cytology
  • Mesencephalon/embryology
  • Mesencephalon/metabolism
  • Molecular Sequence Data
  • Mutation/genetics
  • Neurons/cytology
  • Neurons/metabolism
  • Phylogeny
  • Prosencephalon/cytology
  • Prosencephalon/embryology
  • Prosencephalon/metabolism
  • Proteins/genetics
  • Proteins/isolation & purification*
  • Proteins/metabolism
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism*
  • Signal Transduction/genetics
  • Telencephalon/cytology
  • Telencephalon/embryology*
  • Telencephalon/metabolism
  • Transforming Growth Factor beta*
  • Wnt Proteins
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/isolation & purification*
(all 40)
PubMed
12160744 Full text @ Neuron
Abstract
Cells at the anterior boundary of the neural plate (ANB) can induce telencephalic gene expression when transplanted to more posterior regions. Here, we identify a secreted Frizzled-related Wnt antagonist, Tlc, that is expressed in ANB cells and can cell nonautonomously promote telencephalic gene expression in a concentration-dependent manner. Moreover, abrogation of Tlc function compromises telencephalic development. We also identify Wnt8b as a locally acting modulator of regional fate in the anterior neural plate and a likely target for antagonism by Tlc. Finally, we show that tlc expression is regulated by signals that establish early antero-posterior and dorso-ventral ectodermal pattern. From these studies, we propose that local antagonism of Wnt activity within the anterior ectoderm is required to establish the telencephalon.
Genes / Markers
Marker Marker Type Name
dkk1bGENEdickkopf WNT signaling pathway inhibitor 1b
emx3GENEempty spiracles homeobox 3
fgf8aGENEfibroblast growth factor 8a
hesx1GENEHESX homeobox 1
pax2aGENEpaired box 2a
tlcGENEsecreted frizzled-related protein
wnt1GENEwingless-type MMTV integration site family, member 1
wnt8aGENEwingless-type MMTV integration site family, member 8a
wnt8bGENEwingless-type MMTV integration site family, member 8b
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Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
dkk1bMO3-dkk1bMRPHLNO
tlcMO1-tlcMRPHLNO
wnt1MO3-wnt1MRPHLNO
wnt8aMO1-wnt8aMRPHLNO
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Fish
Antibodies
Orthology
Engineered Foreign Genes
No data available
Mapping