PUBLICATION
Cloning of zebrafish ovarian carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase and characterization of its spatial and temporal expression
- Authors
- Wang, Y. and Ge, W.
- ID
- ZDB-PUB-020913-2
- Date
- 2002
- Source
- General and comparative endocrinology 127(3): 209-216 (Journal)
- Registered Authors
- Keywords
- carbonyl reductase-like; 20b-hydroxysteroid dehydrogenase; (20b-HSD); gonadotropin; activin; ovary; oocyte maturation; zebrafish
- MeSH Terms
-
- Alcohol Oxidoreductases/analysis
- Alcohol Oxidoreductases/chemistry
- Alcohol Oxidoreductases/genetics*
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular*
- Cortisone Reductase/analysis
- Cortisone Reductase/chemistry
- Cortisone Reductase/genetics*
- DNA/chemistry
- Female
- Gene Expression*
- Gills/enzymology
- Kidney/enzymology
- Male
- Molecular Sequence Data
- Ovarian Follicle/enzymology
- Ovary/enzymology*
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology
- Testis/enzymology
- Tissue Distribution
- Zebrafish/genetics*
- PubMed
- 12225761 Full text @ Gen. Comp. Endocrinol.
Citation
Wang, Y. and Ge, W. (2002) Cloning of zebrafish ovarian carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase and characterization of its spatial and temporal expression. General and comparative endocrinology. 127(3):209-216.
Abstract
20beta-Hydroxysteroid dehydrogenase (20beta-HSD) is a crucial enzyme that converts 17alpha-hydroxyprogesterone to 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP), which triggers oocyte maturation in most teleost fish. A full-length cDNA for a carbonyl reductase-like 20beta-HSD (CR/20beta-HSD) has been cloned from the zebrafish ovary. Although the zebrafish CR/20beta-HSD is expressed in all of the tissues tested, it is predominantly expressed in the ovary, testis, kidney, and gill. In the ovary, the enzyme was shown to be expressed in the follicle cells and its expression appeared to be constitutive. No significant difference was noticed in the level of CR/20beta-HSD expression among follicles of different stages. Furthermore, analysis of the ovarian samples taken at different times before spawning showed no significant change of the enzyme expression. In agreement with these results, treatment of the cultured zebrafish ovarian follicle cells with gonadotropin and activin had little effect on the expression of the enzyme. Taken together, these results point to the possibility that the gonadotropin-induced DHP production and final oocyte maturation in the zebrafish may not involve significant change of CR/20beta-HSD expression as evidenced in the salmonids, or that there might be other isoforms of 20beta-HSD whose expression is tightly controlled by endocrine and paracrine factors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping