PUBLICATION
FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain
- Authors
- Maves, L., Jackman, W., and Kimmel, C.B.
- ID
- ZDB-PUB-020806-4
- Date
- 2002
- Source
- Development (Cambridge, England) 129: 3825-3837 (Journal)
- Registered Authors
- Jackman, William (Bill), Kimmel, Charles B., Maves, Lisa
- Keywords
- hindbrain; rhombomere; organizer; FGF3; FGF8; acerebellar; valentino; krox-20; zebrafish
- MeSH Terms
-
- Animals
- Base Sequence
- Body Patterning
- Brain Tissue Transplantation
- DNA-Binding Proteins/genetics
- Early Growth Response Protein 2
- Fibroblast Growth Factor 3
- Fibroblast Growth Factor 8
- Fibroblast Growth Factors/antagonists & inhibitors
- Fibroblast Growth Factors/genetics
- Fibroblast Growth Factors/physiology*
- Gene Expression Regulation, Developmental
- MafB Transcription Factor
- Models, Neurological
- Mosaicism
- Nerve Tissue Proteins/genetics
- Neurons/cytology
- Oligodeoxyribonucleotides, Antisense/genetics
- Oligodeoxyribonucleotides, Antisense/pharmacology
- Proto-Oncogene Proteins/antagonists & inhibitors
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/physiology*
- Rhombencephalon/embryology*
- Signal Transduction
- Transcription Factors/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Zebrafish Proteins*
- PubMed
- 12135921 Full text @ Development
Citation
Maves, L., Jackman, W., and Kimmel, C.B. (2002) FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain. Development (Cambridge, England). 129:3825-3837.
Abstract
The segmentation of the vertebrate hindbrain into rhombomeres is highly conserved, but how early hindbrain patterning is established is not well understood. We show that rhombomere 4 (r4) functions as an early- differentiating signaling center in the zebrafish hindbrain. Time-lapse analyses of zebrafish hindbrain development show that r4 forms first and hindbrain neuronal differentiation occurs first in r4. Two signaling molecules, FGF3 and FGF8, which are both expressed early in r4, are together required for the development of rhombomeres adjacent to r4, particularly r5 and r6. Transplantation of r4 cells can induce expression of r5/r6 markers, as can misexpression of either FGF3 or FGF8 . Genetic mosaic analyses also support a role for FGF signaling acting from r4. Taken together, our findings demonstrate a crucial role for FGF -mediated inter-rhombomere signaling in promoting early hindbrain patterning and underscore the significance of organizing centers in patterning the vertebrate neural plate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping