PUBLICATION
T-box gene tbx5 is essential for formation of the pectoral limb bud
- Authors
- Ahn, D.-G., Kourakis, M.J., Rohde, L.A., Silver, L.M., and Ho, R.K.
- ID
- ZDB-PUB-020624-8
- Date
- 2002
- Source
- Nature 417(6890): 754-758 (Journal)
- Registered Authors
- Ahn, Dae-gwon, Ho, Robert K., Rohde, Laurel
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Biomarkers/analysis
- Cartilage/growth & development
- Cartilage/metabolism
- Down-Regulation
- Gene Expression Regulation, Developmental
- Genes, Essential
- Larva/genetics
- Larva/growth & development
- Limb Buds/cytology
- Limb Buds/embryology*
- Limb Buds/metabolism
- Mutation
- Oligonucleotides, Antisense/genetics
- Phenotype
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/growth & development
- PubMed
- 12066188 Full text @ Nature
Citation
Ahn, D.-G., Kourakis, M.J., Rohde, L.A., Silver, L.M., and Ho, R.K. (2002) T-box gene tbx5 is essential for formation of the pectoral limb bud. Nature. 417(6890):754-758.
Abstract
The T-box genes Tbx4 and Tbx5 have been shown to have key functions in the specification of the identity of the vertebrate forelimb (Tbx5) and hindlimb (Tbx4). Here we show that in zebrafish, Tbx5 has an additional early function that precedes the formation of the limb bud itself. Functional knockdown of zebrafish tbx5 through the use of an antisense oligonucleotide resulted in a failure to initiate fin bud formation, leading to the complete loss of pectoral fins. The function of the tbx5 gene in the development of zebrafish forelimbs seems to involve the directed migration of individual lateral-plate mesodermal cells into the future limb-bud-producing region. The primary defect seen in the tbx5- knockdown phenotype is similar to the primary defects described in known T-box-gene mutants such as the spadetail mutant of zebrafish and the Brachyury mutant of the mouse, which both similarly exhibit an altered migration of mesodermal cells. A common function for many of the T-box genes might therefore be in mediating the proper migration and/or changes in adhesive properties of early embryonic cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping