PUBLICATION
Distinct expression patterns of two zebrafish homologues of the human APP gene during embryonic development
- Authors
- Musa, A., Lehrach, H., and Russo, V.E.A.
- ID
- ZDB-PUB-020219-6
- Date
- 2001
- Source
- Development genes and evolution 211(11): 563-567 (Journal)
- Registered Authors
- Lehrach, Hans, Musa, Alberto
- Keywords
- zebrafish; Alzheimer's disease; amyloid protein precursor; appa; appb
- MeSH Terms
-
- Amyloid beta-Protein Precursor/genetics*
- Amyloidogenic Proteins/genetics*
- Animals
- Embryo, Nonmammalian/metabolism
- Gene Expression
- Humans
- Sequence Homology
- Sequence Homology, Amino Acid
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish Proteins/genetics*
- PubMed
- 11862463 Full text @ Dev. Genes Evol.
Citation
Musa, A., Lehrach, H., and Russo, V.E.A. (2001) Distinct expression patterns of two zebrafish homologues of the human APP gene during embryonic development. Development genes and evolution. 211(11):563-567.
Abstract
The human amyloid protein precursor (APP) gene correlates with early onset of Alzheimer's disease in humans. We have identified two APP homologues in zebrafish, which we call appa and appb. They show a high degree of identity to human APP particularly in the APP42 and the transmembrane domain. Widespread expression of both appa and appb was detected from mid-gastrulation until the bud stage. During segmentation, the two genes diverged in their pattern of expression: at 14 h post-fertilisation (hpf) and 18 hpf both genes were expressed rostrally in the prospective CNS, but only appa was found caudally in the paraxial segmental plate and presomitic mesoderm, excluding the midline. In contrast, appb was found caudally in the neural rod at 14 hpf and the developing spinal cord at 18 hpf. Later, at 24 hpf both genes shared common expression domains, namely the telencephalon, the ventral diencephalon, the trigeminal ganglia, and the posterior lateral line ganglia. Unique expression domains for appa were the lens, the otic vesicles and the somites, while appb was expressed in a serially repeated set of nuclei within the hindbrain, the ventral mesencephalon and the motoneurones of the developing spinal cord.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping