PUBLICATION
The antigen receptor (NCCRP-1) on catfish and zebrafish nonspecific cytotoxic cells belongs to a new gene family characterized by an F-Box-Associated domain
- Authors
- Jaso-Friedmann, L., Peterson, D.S., Gonzalez, D.S., and Evans, D.L.
- ID
- ZDB-PUB-020219-16
- Date
- 2002
- Source
- Journal of molecular evolution 54(3): 386-395 (Journal)
- Registered Authors
- Jaso-Friedmann, Liliana
- Keywords
- zebrafish; nonspecific cytotoxic cells; antigen-binding domain; catfish; expressed sequence tags; F-box; F-box-associated domain
- MeSH Terms
-
- Amino Acid Motifs/genetics
- Amino Acid Sequence
- Animals
- Base Sequence
- Catfishes/genetics*
- Catfishes/immunology
- Consensus Sequence/genetics
- Evolution, Molecular
- Killer Cells, Natural/immunology*
- Molecular Sequence Data
- Phylogeny
- Protein Structure, Tertiary/genetics
- Receptors, Antigen/genetics*
- Sequence Alignment
- Sequence Analysis, DNA
- Zebrafish/genetics*
- Zebrafish/immunology
- PubMed
- 11847564 Full text @ J. Mol. Evol.
Citation
Jaso-Friedmann, L., Peterson, D.S., Gonzalez, D.S., and Evans, D.L. (2002) The antigen receptor (NCCRP-1) on catfish and zebrafish nonspecific cytotoxic cells belongs to a new gene family characterized by an F-Box-Associated domain. Journal of molecular evolution. 54(3):386-395.
Abstract
The catfish nonspecific cytotoxic cell receptor protein (NCCRP-1) provides an important function in target cell recognition and activation of cytotoxicity. This report identifies and characterizes a zebrafish orthologue of the catfish NCCRP-1. The zebrafish NCCRP-1 cDNA contains an open reading frame that encodes a predicted protein of 237 amino acids with a MW of 27 kDa and a pI of 5.5. Sequence similarities comparisons show that the NCCRP-1 receptors from these two phylogenetically distant species share a high degree of identity. These results suggested that NCCRP-1 performs a crucial function in innate immunity in teleosts. Further, a zebrafish 17-mer peptide corresponding to the catfish NCCRP-1 antigen-binding domain inhibited (catfish) cytotoxicity toward conventional tumor target cells (HL-60). These data appeared to indicate that the zebrafish NCCRP-1 protein may function as an antigen recognition molecule and, as such, may participate in innate immunity in teleosts. A homology search of the zebrafish NCCRP-1 protein revealed that it shares a significant level of identity with another group of proteins belonging to an F-box subfamily. These proteins share an F-box domain in the N terminus (not present in NCCRP-1) and an extremely conserved C-terminal region that has been termed the F-box-associated domain (FBA). The FBA is currently of unknown function. A new gene family is proposed in this work, based on similarities in the FBA sequences with the catfish and zebrafishNCCRP-1 peptides. This new gene family includes several F-box domain-containing proteins and a predicted C. elegans protein.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping