PUBLICATION
Novel form of fibronectin from zebrafish mediates infectious hematopoietic necrosis virus infection
- Authors
- Liu, X. and Collodi, P.
- ID
- ZDB-PUB-020102-3
- Date
- 2002
- Source
- Journal of virology 76(2): 492-498 (Journal)
- Registered Authors
- Collodi, Paul, Liu, Xiangyu
- Keywords
- nicotinic acetylcholine receptor, rabies virus, fish rhabdovirus, phosphatidylserine-binding, synthetic peptides, rainbow trout, glycoprotein, protein, embryos, domain
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cell Line
- Cytopathogenic Effect, Viral/drug effects
- Fibronectins/chemistry*
- Fibronectins/genetics
- Fibronectins/metabolism*
- Fibronectins/pharmacology
- Gene Expression
- Infectious hematopoietic necrosis virus/drug effects
- Infectious hematopoietic necrosis virus/pathogenicity
- Infectious hematopoietic necrosis virus/physiology*
- Membrane Proteins/chemistry
- Membrane Proteins/genetics
- Membrane Proteins/metabolism
- Membrane Proteins/pharmacology
- Molecular Sequence Data
- Receptors, Virus/chemistry
- Receptors, Virus/genetics
- Receptors, Virus/isolation & purification
- Receptors, Virus/metabolism
- Transfection
- Viral Plaque Assay
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish/virology*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Zebrafish Proteins/pharmacology
- PubMed
- 11752139 Full text @ J. Virol.
Citation
Liu, X. and Collodi, P. (2002) Novel form of fibronectin from zebrafish mediates infectious hematopoietic necrosis virus infection. Journal of virology. 76(2):492-498.
Abstract
The presence of a novel form of zebrafish fibronectin (FN2) on the cell surface increased the cell's susceptibility to infection by infectious hematopoietic necrosis virus (IHNV). Unlike other fibronectins, FN2 possesses a truncated structure and accumulates on the cell surface instead of in the extracellular matrix. Fish embryo cells expressing recombinant FN2 were more susceptible to IHNV infection, with a greater percentage of cells exhibiting cytopathic effect (CPE) compared to nontransfected control cells. Incubation of nontransfected cells with soluble recombinant FN2 increased IHNV infection, as measured by plaque assay. The number of plaques increased in correlation with the amount of protein added and the length of time that cells were incubated with the protein. Incubation of IHNV with soluble FN2 before addition to cells also increased infection. FN2 immobilized on the culture surface inhibited IHNV infection. The results indicate that FN2 present on the cell surface is able to mediate IHNV attachment and cell entry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping