PUBLICATION
Growth and function of the embryonic heart depend upon the cardiac-specific L-type calcium channel alpha1 subunit
- Authors
- Rottbauer, W., Baker, K., Wo, Z.G., Mohideen, M.A., Cantiello, H.F., and Fishman, M.C.
- ID
- ZDB-PUB-011205-2
- Date
- 2001
- Source
- Developmental Cell 1(2): 265-275 (Journal)
- Registered Authors
- Fishman, Mark C., Mohideen, Manzoor Pallithotangal, Rottbauer, Wolfgang, Wo, Galen
- Keywords
- none
- MeSH Terms
-
- Alleles
- Amino Acid Sequence
- Animals
- Atrial Fibrillation
- Calcium/metabolism
- Calcium Channels, L-Type/chemistry*
- Calcium Channels, L-Type/physiology*
- Gene Library
- Heart/embryology*
- In Situ Hybridization
- Microscopy, Electron
- Models, Biological
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Myocardium/cytology
- Myocardium/metabolism
- Pancreas/metabolism
- Patch-Clamp Techniques
- Protein Structure, Tertiary
- RNA, Messenger/metabolism
- Sequence Homology, Amino Acid
- Signal Transduction
- Time Factors
- Zebrafish
- PubMed
- 11702785 Full text @ Dev. Cell
Citation
Rottbauer, W., Baker, K., Wo, Z.G., Mohideen, M.A., Cantiello, H.F., and Fishman, M.C. (2001) Growth and function of the embryonic heart depend upon the cardiac-specific L-type calcium channel alpha1 subunit. Developmental Cell. 1(2):265-275.
Abstract
The heart must function from the moment of its embryonic assembly, but the molecular underpinnings of the first heart beat are not known, nor whether function determines form at this early stage. Here, we find by positional cloning that the embryonic lethal island beat (isl) mutation in zebrafish disrupts the alpha1 C L-type calcium channel subunit (C-LTCC). The isl atrium is relatively normal in size, and individual cells contract chaotically, in a pattern resembling atrial fibrillation. The ventricle is completely silent. Unlike another mutation with a silent ventricle, isl fails to acquire the normal number of myocytes. Thus, calcium signaling via C-LTCC can regulate heart growth independently of contraction, and plays distinctive roles in fashioning both form and function of the two developing chambers.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping