PUBLICATION
Critical role of biklf in erythroid cell differentiation in zebrafish
- Authors
- Kawahara, A. and Dawid, I.B.
- ID
- ZDB-PUB-010905-2
- Date
- 2001
- Source
- Current biology : CB 11(17): 1353-1357 (Journal)
- Registered Authors
- Dawid, Igor B., Kawahara, Atsuo
- Keywords
- none
- MeSH Terms
-
- Animals
- Basic Helix-Loop-Helix Transcription Factors
- Cell Differentiation
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism
- Erythroid Precursor Cells/cytology*
- Erythroid-Specific DNA-Binding Factors
- GATA1 Transcription Factor
- Globins/genetics
- Globins/metabolism
- Kruppel-Like Transcription Factors
- Proto-Oncogene Proteins*
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription Factors/physiology*
- Zebrafish
- Zebrafish Proteins*
- Zinc Fingers/genetics
- Zinc Fingers/physiology*
- PubMed
- 11553329 Full text @ Curr. Biol.
Citation
Kawahara, A. and Dawid, I.B. (2001) Critical role of biklf in erythroid cell differentiation in zebrafish. Current biology : CB. 11(17):1353-1357.
Abstract
Hematopoietic cells arise from ventral mesoderm in different vertebrates, but the mechanisms through which various factors contribute to the hematopoietic processes, including erythrogenesis, remain incompletely understood. The Kruppel-like transcription factor Biklf is preferentially expressed in blood islands throughout zebrafish embryogenesis, marking the region of future erythropoiesis [1]. In this paper, we show that expression of biklf is significantly suppressed in the blood-less mutants vampire and m683 in which primitive hematopoiesis is impaired. Knockdown of biklf using morpholino-based antisense oligonucleotides (biklf-MO) led to a potent reduction in the number of circulating blood cells and deficiency in hemoglobin production. Consistently, we found that the expression of beta(e3)globin is strongly suppressed in biklf-MO-injected embryos, while gata1 expression is partly inhibited at the 10-somite stage. In addition, analysis of reporter constructs driven by the GATA1 and beta-globin promoters showed that Biklf can positively regulate both genes. These results indicate that Biklf is required for erythroid cell differentiation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping