PUBLICATION

astray, a zebrafish roundabout homolog required for retinal axon guidance

Authors
Fricke, C., Lee, J.S., Geiger-Rudolph, S., Bonhoeffer, F., and Chien, C.-B.
ID
ZDB-PUB-010423-3
Date
2001
Source
Science (New York, N.Y.)   292(5516): 507-510 (Journal)
Registered Authors
Bonhoeffer, Friedrich, Chien, Chi-Bin, Fricke, Cornelia, Geiger-Rudolph, Silke, Lee, Jeong-Soo
Keywords
none
MeSH Terms
  • Alleles
  • Animals
  • Axons/physiology*
  • Body Patterning
  • Chromosome Mapping
  • Crosses, Genetic
  • Eye/embryology
  • Eye/transplantation
  • Female
  • Gene Expression Regulation, Developmental
  • Genes
  • In Situ Hybridization
  • Male
  • Mutation
  • Nerve Tissue Proteins/genetics*
  • Nerve Tissue Proteins/physiology
  • Phenotype
  • Receptors, Immunologic/genetics*
  • Receptors, Immunologic/physiology*
  • Retina/embryology
  • Retina/metabolism
  • Retinal Ganglion Cells/metabolism
  • Retinal Ganglion Cells/physiology*
  • Superior Colliculi/cytology
  • Superior Colliculi/embryology*
  • Visual Pathways/embryology
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins
(all 29)
PubMed
11313496 Full text @ Science
Abstract
As growing retinotectal axons navigate from the eye to the tectum, they sense guidance molecules distributed along the optic pathway. Mutations in the zebrafish astray gene severely disrupt retinal axon guidance, causing anterior-posterior pathfinding defects, excessive midline crossing, and defasciculation of the retinal projection. Eye transplantation experiments show that astray function is required in the eye. We identify astray as zebrafish robo2, a member of the Roundabout family of axon guidance receptors. Retinal ganglion cells express robo2 as they extend axons. Thus, robo2 is required for multiple axon guidance decisions during establishment of the vertebrate visual projection
Genes / Markers
Marker Marker Type Name
robo2GENEroundabout, axon guidance receptor, homolog 2 (Drosophila)
z13822SSLPZ13822
z26441SSLPZ26441
z7381SSLPZ7381
1 - 4 of 4
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Figures
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
robo2WTstandard conditionsPrim-5ISH
robo2WTstandard conditionsPrim-25ISH
robo2WTstandard conditionsPrim-15ISH
robo2WTstandard conditionsProtruding-mouthISH
1 - 4 of 4
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
te284
    		Point Mutation
    ti272z
      		Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      robo2te284/te284
      robo2ti272z/ti272z
      WT
      1 - 3 of 3
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      Antibodies
      No data available
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping
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      Citation
      Fricke, C., Lee, J.S., Geiger-Rudolph, S., Bonhoeffer, F., and Chien, C.-B. (2001) astray, a zebrafish roundabout homolog required for retinal axon guidance. Science (New York, N.Y.). 292(5516):507-510.