PUBLICATION
Expression of the chaperonin 10 gene during zebrafish development
- Authors
- Martin, C.C., Tang, P., Barnardo, G., and Krone, P.H.
- ID
- ZDB-PUB-010416-2
- Date
- 2001
- Source
- Cell stress & chaperones 6(1): 38 (Journal)
- Registered Authors
- Krone, Patrick H., Martin, C. Cristofre, Tang, Ping-tao
- Keywords
- none
- MeSH Terms
-
- Animals
- Chaperonin 10/blood
- Chaperonin 10/genetics*
- Cloning, Molecular
- Female
- Gene Expression Regulation, Developmental*
- Heat-Shock Response/physiology
- Hot Temperature
- Male
- Pregnancy
- RNA, Messenger/analysis
- Species Specificity
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 11525241 Full text @ Cell Stress Chaperones
Citation
Martin, C.C., Tang, P., Barnardo, G., and Krone, P.H. (2001) Expression of the chaperonin 10 gene during zebrafish development. Cell stress & chaperones. 6(1):38.
Abstract
We have isolated a cDNA encoding chaperonin 10 (cpn10) from the zebrafish. Using northern, western, and in situ hybridization analysis, we observed that the cpn10 gene is expressed uniformly and ubiquitously throughout embryonic development of the zebrafish. Upregulation of cpn10 expression was observed following exposure of zebrafish embryos to a heat shock of 1 hour at 37°C compared to control embryos raised at 27°C. The extracellular form of Cpn10 called early pregnancy factor (EPF), found in the serum of pregnant mammals, was not detected in the serum of either male or female zebrafish. These expression studies suggest that Cpn10 plays a general role in zebrafish development as well as being consistent with the hypothesis that EPF is involved in the embryo implantation process in mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping