PUBLICATION
Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor a in zebrafish
- Authors
- Haire, R.N., Rast, J.P., Litman, R.T., and Litman, G.W.
- ID
- ZDB-PUB-001017-4
- Date
- 2000
- Source
- Immunogenetics 51(11): 915-923 (Journal)
- Registered Authors
- Haire, Robert, Litman, Gary W.
- Keywords
- DNA sequence; developmental model; immunoglobulin diversity; short-primer PCR; teleost immunoglobulin
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA, Complementary
- Genes, T-Cell Receptor alpha*
- Immunoglobulin Constant Regions/classification
- Immunoglobulin Constant Regions/genetics*
- Immunoglobulin Isotypes/classification
- Immunoglobulin Isotypes/genetics
- Immunoglobulin Joining Region/classification
- Immunoglobulin Joining Region/genetics*
- Immunoglobulin Light Chains/classification
- Immunoglobulin Light Chains/genetics*
- Immunoglobulin Variable Region/classification
- Immunoglobulin Variable Region/genetics*
- Molecular Sequence Data
- Phylogeny
- Sequence Homology, Amino Acid
- Zebrafish/genetics*
- Zebrafish/immunology
- PubMed
- 11003385 Full text @ Immunogenetics
Citation
Haire, R.N., Rast, J.P., Litman, R.T., and Litman, G.W. (2000) Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor a in zebrafish. Immunogenetics. 51(11):915-923.
Abstract
The zebrafish (Danio rerio) has become a significant model for understanding the developmental regulation of gene expression and holds considerable potential for characterizing the development of the immune system. Using a number of different approaches, including heterologous hybridization and short-primer PCR, cDNAs for three different classes of light-chain genes were identified and characterized. The zebrafish light chains are similar to trout type 1, trout type 2, and catfish type F, respectively. T-cell antigen receptor alpha (TCRalpha) was also identified and characterized. A high proportion of unusual transcripts including sterile transcripts, germline VJC transcripts, aberrant splice forms, and V-V transcripts were encountered in the immunoglobulin and TCR cDNAs examined. The light-chain and TCRalpha loci each consist of multiple families of V gene segments, apparent even from the small numbers of cDNAs of each isotype sequenced. The gene sequences reported provide an essential set of markers of both B- and T-cell lineages that will facilitate investigations of immune system development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping