PUBLICATION
Isolation of complementary DNAs coding for a receptor for activated C kinase (RACK) from zebrafish (Danio rerio) and tilapia (Oreochromis niloticus): constitutive tissue and developmental expression
- Authors
- Hamilton, L. and Wright, J.M.
- ID
- ZDB-PUB-000622-12
- Date
- 1999
- Source
- Marine biotechnology (New York, N.Y.) 1(3): 279-285 (Journal)
- Registered Authors
- Wright, Jonathan M.
- Keywords
- receptor for activated C kinase (RACK), zebrafish, tilapia, in situ hybridization, development, fish embryos
- MeSH Terms
- none
- PubMed
- 10384002 Full text @ Mar. Biotechnol.
Citation
Hamilton, L. and Wright, J.M. (1999) Isolation of complementary DNAs coding for a receptor for activated C kinase (RACK) from zebrafish (Danio rerio) and tilapia (Oreochromis niloticus): constitutive tissue and developmental expression. Marine biotechnology (New York, N.Y.). 1(3):279-285.
Abstract
We have cloned and sequenced complementary DNAs coding for a receptor for activated C kinase (RACK) from two species of teleost fishes, zebrafish (Danio rerio) and tilapia (Oreochromis niloticus). The tilapia clone is 1063 nucleotides long, and the zebrafish clone is 1069 nucleotides long. Both clones contain an open reading frame coding for the complete RACK protein of 317 amino acids. Northern hybridization analysis using these clones as probes detected a 1.2-kb band, indicating that these are nearly full-length cDNA clones. In tilapia, RACK messenger RNA was expressed in all tissues examined. In situ hybridization detected the presence of mRNA for this RACK sequence in unfertilized eggs and embryos (development up to 24 hours) from zebrafish.
http://link.springer.de/link/service/journals/10126/papers/1n3p279.pdf
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping