ZFIN ID: ZDB-PERS-970128-5 |
Hauptmann, Giselbert
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BIOGRAPHY AND RESEARCH INTERESTS
In situ mRNA detection and visualization in neural tissue
Evolution and development of vertebrate forebrain organization
Development and function of neuroendocrine hypothalamus
Development and function of corticotropin-releasing-hormone neuronal
systems
Evolution and development of vertebrate forebrain organization
Development and function of neuroendocrine hypothalamus
Development and function of corticotropin-releasing-hormone neuronal
systems
PUBLICATIONS
NON-ZEBRAFISH PUBLICATIONS
Hauptmann, G. & Gerster, T.
"Multicolor whole-mount in situ hybridization to Drosophila embryos"
Dev. Genes Evol. 1996 Dec (206): 292-295
ABSTRACT
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin,
respectively, this protocol allows the detection in three colors of RNAs derived from three different genes. Hybridized probes are detected by consecutive
staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by
low pH washes.