Person
de Vrieze, Erik
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Biography and Research Interest
Current project:
Usher Syndrome, Functional Genomics and Therapeutics
Usher syndrome is genetically and clinically heterogeneous. The syndrome combines hearing impairment, retinitis pigmentosa and in part of the patients vestibular impairment. Usher syndrome type IIa (USH2a), the most common type, is caused by mutations in the USH2A gene. Other genes involved in Usher syndrome are MYO7A, USH1C, CDH23, PCDH15 and USH1G (type I), GPR98 and DFNB31 (type II) and USH3A (type III). Also, defects in the USH2A gene are an important cause of non-syndromic retinitis pigmentosa. Usher proteins co-function in a protein network which explains why defects in genes encoding functionally different proteins are causative for one and the same disorder. We have a specific interest in the USH2A protein and whirlin. Recently, we have shown that NINL, which we identified as an interaction partner of USH2A, connects proteins involved in different retinal degeneration disorders (Usher syndrome, Leber congenital amaurosis and Bardet Biedl syndrome). This suggests an overlap in the molecular pathogenesis of these disorders. Further unravelling of the USH protein network is ongoing by using yeast two-hybrid screening and tandem affinity purification (TAP). The mechanisms of hearing impairment in Usher syndrome are at least partly understood. Those of retinal degeneration in Usher syndrome are largely unknown but the localization of the Usher proteins in the region of the connecting cilium of photoreceptor cells suggests that dysfunction of this structure which heavily functions in transport, is involved. To further study the function of Usher proteins and the mechanisms of retinal degeneration we are introducing zebrafish as a model organism. In this model we shall address therapeutic strategies for USH2A-associated retinal degeneration.
Additional research interests:
Endocrinology and bone physiology
I have generated zebrafish models to study endocrine diseases. A good example my assay to study the (anti) osteogenic potential of hormones and drugs based on zebrafish scales. A single fish scale, highly similar to human bone in terms of cell population and matrix, can be cultured ex-vivo as a complete bone unit. This offers many advantages over the use of cell culture systems. Furthermore, the large amount of scales on a single zebrafish allows a drastic reduction of experimental animals compared to mammalian tissue culture.
My interests in endocrinology further extend to thyroidology, stress and metabolism.
Physiology and pathophysiology of inherited retinal diseases
Besides Usher syndrome, I have been involved in the generation and validation of zebrafish models for other hereditary retinal diseases. These models are used to assess the pathogenicity of newly discovered candidate disease genes
Usher Syndrome, Functional Genomics and Therapeutics
Usher syndrome is genetically and clinically heterogeneous. The syndrome combines hearing impairment, retinitis pigmentosa and in part of the patients vestibular impairment. Usher syndrome type IIa (USH2a), the most common type, is caused by mutations in the USH2A gene. Other genes involved in Usher syndrome are MYO7A, USH1C, CDH23, PCDH15 and USH1G (type I), GPR98 and DFNB31 (type II) and USH3A (type III). Also, defects in the USH2A gene are an important cause of non-syndromic retinitis pigmentosa. Usher proteins co-function in a protein network which explains why defects in genes encoding functionally different proteins are causative for one and the same disorder. We have a specific interest in the USH2A protein and whirlin. Recently, we have shown that NINL, which we identified as an interaction partner of USH2A, connects proteins involved in different retinal degeneration disorders (Usher syndrome, Leber congenital amaurosis and Bardet Biedl syndrome). This suggests an overlap in the molecular pathogenesis of these disorders. Further unravelling of the USH protein network is ongoing by using yeast two-hybrid screening and tandem affinity purification (TAP). The mechanisms of hearing impairment in Usher syndrome are at least partly understood. Those of retinal degeneration in Usher syndrome are largely unknown but the localization of the Usher proteins in the region of the connecting cilium of photoreceptor cells suggests that dysfunction of this structure which heavily functions in transport, is involved. To further study the function of Usher proteins and the mechanisms of retinal degeneration we are introducing zebrafish as a model organism. In this model we shall address therapeutic strategies for USH2A-associated retinal degeneration.
Additional research interests:
Endocrinology and bone physiology
I have generated zebrafish models to study endocrine diseases. A good example my assay to study the (anti) osteogenic potential of hormones and drugs based on zebrafish scales. A single fish scale, highly similar to human bone in terms of cell population and matrix, can be cultured ex-vivo as a complete bone unit. This offers many advantages over the use of cell culture systems. Furthermore, the large amount of scales on a single zebrafish allows a drastic reduction of experimental animals compared to mammalian tissue culture.
My interests in endocrinology further extend to thyroidology, stress and metabolism.
Physiology and pathophysiology of inherited retinal diseases
Besides Usher syndrome, I have been involved in the generation and validation of zebrafish models for other hereditary retinal diseases. These models are used to assess the pathogenicity of newly discovered candidate disease genes
Non-Zebrafish Publications
Metz, J. R., de Vrieze, E., Lock, E.-J., Schulten, I. E. and Flik, G. (2012), Elasmoid scales of fishes as model in biomedical bone research. Journal of Applied Ichthyology, 28: 382-387.
de Vrieze, E., Heijnen, L., Metz, J. R. and Flik, G. (2012), Evidence for a hydroxyapatite precursor in regenerating cyprinid scales. Journal of Applied Ichthyology, 28: 388-392.
Gorissen, M., de Vrieze, E., Flik, G. & Huising, M. O. (2011). STAT genes display differential evolutionary rates that correlate with their roles in the endocrine and immune system. Journal of Endocrinology 209, 175-184.
E. De Vrieze, J.R. Metz, J.W. Von den Hoff, G. Flik, ALP, TRAcP and cathepsin K in elasmoid scales: a role in mineral metabolism?, Journal of Applied Ichthyology 26 (2010) 210-213.