Person
Tiso, Natascia
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Biography and Research Interest
Personal data
Nationality: Italian
Foreign languages: English, French.
Qualifications
July 1989: School-leaving Certificate (Scientific High School 'E. Fermi', Padova); full marks (60 / 60).
13th of July 1994: Graduation on Biological Sciences (Biomolecular Course), University of Padova;
first-class honours degree (110 / 110 magna cum laude).
January 1996: Official State Qualification as a Professional Biologist, University of Padova.
15th of January - 20th of December 1997: Visiting Scientist at the Human Genetics Laboratory (Supervisor: Dr. J. M. Rommens - discoverer of the CF gene), Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
May 1998: PhD in Genetics, Universities of Bologna, Ferrara and Padova.
September 1998: Two-year Post-doctoral Fellowship at the Human Genetics Laboratory, University of Padova.
December 2000: Three-year EU contract (Research Assistant) at the Laboratory of Developmental Genetics, University of Padova.
March 2004: Four-year EU contract (Research Assistant) in the above-mentioned work place.
Aug-Dec 2005: EU-Screen at the Max Planck Institute of Tuebingen - Germany (Supervisor: Prof. C. Nuesslein-Volhard - Nobel Laureate).
October 2007-August 2015: Assistant Professor in Genetics, University of Padova, Italy.
September 2015-present: Associate Professor in Applied Biology, University of Padova, Italy
Research activity
Prof. N. Tiso's research activity dealt with the localization and the characterization of human disease genes by linkage analysis, positional cloning, mutation screening and protein interaction studies. In particular, Prof. N. Tiso worked on one form of Arrhythmogenic Right Ventricular Cardiomyopathy, whose locus (ARVD2) had been previously mapped in Prof. Danieli's laboratory, and she identified the causative gene (RYR2). Prof. N. Tiso' work experience also included systematic mapping of ESTs and human genes by monochromosomal and radiation hybrid panels; cDNA selection from specific sub-chromosomal regions and reconstruction of the genomic structure of human genes by Long-Distance, Alu and in silico PCR. Prof. N. Tiso's present research interests mainly concern signaling pathway analysis and disease modeling in zebrafish, with particular attention to the neural, pancreatic, thyroidal, cardiac and mitochondrial compartment. These studies are carried out by mRNA and morpholino microinjection, microarray/RNAseq/RealTime RT-PCR strategies, single and double whole mount in situ hybridization in zebrafish embryos, xeno-grafts, Crispr/Cas9 genome editing, analysis of mutants and production of pathway reporter transgenic lines.
Nationality: Italian
Foreign languages: English, French.
Qualifications
July 1989: School-leaving Certificate (Scientific High School 'E. Fermi', Padova); full marks (60 / 60).
13th of July 1994: Graduation on Biological Sciences (Biomolecular Course), University of Padova;
first-class honours degree (110 / 110 magna cum laude).
January 1996: Official State Qualification as a Professional Biologist, University of Padova.
15th of January - 20th of December 1997: Visiting Scientist at the Human Genetics Laboratory (Supervisor: Dr. J. M. Rommens - discoverer of the CF gene), Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
May 1998: PhD in Genetics, Universities of Bologna, Ferrara and Padova.
September 1998: Two-year Post-doctoral Fellowship at the Human Genetics Laboratory, University of Padova.
December 2000: Three-year EU contract (Research Assistant) at the Laboratory of Developmental Genetics, University of Padova.
March 2004: Four-year EU contract (Research Assistant) in the above-mentioned work place.
Aug-Dec 2005: EU-Screen at the Max Planck Institute of Tuebingen - Germany (Supervisor: Prof. C. Nuesslein-Volhard - Nobel Laureate).
October 2007-August 2015: Assistant Professor in Genetics, University of Padova, Italy.
September 2015-present: Associate Professor in Applied Biology, University of Padova, Italy
Research activity
Prof. N. Tiso's research activity dealt with the localization and the characterization of human disease genes by linkage analysis, positional cloning, mutation screening and protein interaction studies. In particular, Prof. N. Tiso worked on one form of Arrhythmogenic Right Ventricular Cardiomyopathy, whose locus (ARVD2) had been previously mapped in Prof. Danieli's laboratory, and she identified the causative gene (RYR2). Prof. N. Tiso' work experience also included systematic mapping of ESTs and human genes by monochromosomal and radiation hybrid panels; cDNA selection from specific sub-chromosomal regions and reconstruction of the genomic structure of human genes by Long-Distance, Alu and in silico PCR. Prof. N. Tiso's present research interests mainly concern signaling pathway analysis and disease modeling in zebrafish, with particular attention to the neural, pancreatic, thyroidal, cardiac and mitochondrial compartment. These studies are carried out by mRNA and morpholino microinjection, microarray/RNAseq/RealTime RT-PCR strategies, single and double whole mount in situ hybridization in zebrafish embryos, xeno-grafts, Crispr/Cas9 genome editing, analysis of mutants and production of pathway reporter transgenic lines.
Non-Zebrafish Publications
Tiso N., Salamon M., Bagattin A., Danieli G.A., Argenton F., Bortolussi M.: 'The binding of the RyR2 calcium channel to its gating protein FKBP12.6 is oppositely affected by ARVD2 and VTSIP mutations'. Biochem. Biophys. Res. Commun. 299: 594-598 (2002).Bauce B., Rampazzo A., Basso C., Bagattin A., Daliento L., Tiso N., Turrini P., Thiene G., Danieli G.A. and Nava A.: 'Screening for RyR2 mutations in families with effort-induced polymorphic ventricular arrhythmias and sudden death: early diagnosis of asymptomatic carriers'. J. Am. Coll. Cardiol 40(2): 341-349 (2002).
De Leo D., Turrina S., Marigo M., Tiso N., Danieli G.A.: 'Italian population data for D1S1656, D3S1358, D8S1132, D10S2325, VWA, FES/FPS, and F13A01'. Forensic Sci Int. Nov 15; 123(1):71-73 (2001).
Turrina S., De Leo D., Marigo M., Tiso N., Danieli G.A.: 'Allele frequency distributions for D1S1656, D8S1132, D10S2325, D18S51, and D21S11 loci in a north Italy population'. J. Forensic Sci. Jan 46(1): 191-192 (2001).
Tiso N., Stephan D.A., Nava A., Bagattin A., Devaney J., Stanchi F., Larderet G., Brahmbhatt B., Brown K., Bauce B., Muriago M., Basso C., Thiene G., Danieli G.A. and Rampazzo A.: 'Identification of mutations in the cardiac Ryanodine Receptor gene in families affected with arrhythmogenic right ventricular cardiomyopathy type 2 (ARVD2)'. Human Molecular Genetics, Feb 1; 10(3):189-194 (2001).
Laitinen P.J., Brown K.M., Piippo K., Swan H., Devaney J.M., Brahmbhatt B., Donarum E.A., Marino M., Tiso N., Viitasalo M., Toivonen L., Stephan D.A., Kontula K.: 'Mutations of the Cardiac Ryanodine Receptor (RyR2) Gene in Familial Polymorphic Ventricular Tachycardia'. Circulation, Jan 30; 103(4): 485-490 (2001).
Priori S.G., Napolitano C., Tiso N., Memmi M., Vignati G., Bloise R., Sorrentino V., Danieli G.A.: 'Mutations in the Cardiac Ryanodine Receptor Gene (hRyR2) underlie catecholaminergic Polymorphic Ventricular Tachycardia'. Circulation, 102: r49-r53 (2000); Circulation, Jan 30, 103(4): 196-200 (2001).
Rampazzo A., Pivotto F., Occhi G., Tiso N., Bortoluzzi S., Rowen L., Hood L., Nava A., and Danieli G.A.: 'Characterization of C14orf4, a novel intronless human gene containing a polyglutamine repeat, mapped to the ARVD1 critical region'. Biochem. Biophys. Res. Commun. Nov 30; 278(3):766-774 (2000).
Stanchi F, Corso V, Scannapieco P, Ievolella C, Negrisolo E, Tiso N, Lanfranchi G, Valle G.: 'TUBA8: A New Tissue-Specific Isoform of alpha-Tubulin That Is Highly Conserved in Human and Mouse'. Biochem. Biophys. Res. Commun. Apr 21; 270(3):1111-1118 (2000).
Rampazzo A., Nava A., Miorin M., Tiso N., Thiene G., Danieli G.A.: 'Molecular genetics of arrhythmogenic right ventricular cardiomyopathy' in From Molecule to Men (Edited by M. Zehender, G. Breithardt, H. Just Steinkopff Darmstadt), pp. 77-80 (2000).
Tiso N., Majetti M., Stanchi F., Rampazzo A., Zimbello,R., Nava A. and Danieli G.A.: 'Fine mapping and genomic structure of ACTN2, the human gene coding for the sarcomeric isoform of alpha-Actinin-2, expressed in skeletal and cardiac muscle'. Biochem. Biophys. Res. Commun. 265, 256-259 (1999).
Bortoluzzi S., Rampoldi L., Simionati B., Zimbello R., Barbon A., d'Alessi F., Tiso N., Pallavicini A., Toppo S., Cannata F., Valle G., Lanfranchi G. and Danieli G.A.: 'A comprehensive high resolution genomic transcript map of human skeletal muscle'. Genome Research, Aug., Vol 8, n. 8: 817-825 (1998).
Rampazzo A., Nava A., Miorin M., Fonderico P., Pope B., Tiso N., Livolsi B., Thiene G.and Danieli G.A: 'ARVD4, a new locus for arrhythmogenic right ventricular cardiomyopathy, maps to chromosome 2 long arm'. Genomics, Oct 15; 45(2) 259-263 (1997).
Rampoldi L., Zimbello R., Tiso N., Pallavicini A., Valle G., Lanfranchi G. and Danieli G.A.: 'Chromosomal localization of four MAPK signalling cascade genes: MEK1, MEK3, MEK4 and MEKK5'. Cytogenetics and Cell Genetics 78: 301-303 (1997).
Valle G., Faulkner G., De Antoni A., Pacchioni B., Pallavicini A., Pandolfo D., Tiso N., Toppo S., Trevisan S., Lanfranchi G.: 'Telethonin, a novel sarcomeric protein of heart and skeletal muscle'. Febbs Lett. Sep 29; 415(2): 163-168 (1997).
Pallavicini A., Zimbello R., Tiso N., Muraro T., Rampoldi L., Bortoluzzi S., Valle G., Lanfranchi G. and Danieli G.A.: 'The preliminary transcript map of a human skeletal muscle'. Human Molecular Genetics, Sep 6 (9): 1445-1450 (1997).
Tiso N., Rampoldi L., Pallavicini A., Zimbello R., Pandolfo D., Valle G., Lanfranchi G. and Danieli G.A.: 'Fine Mapping of Five Human Skeletal Muscle Genes: Alpha-Tropomyosin, Beta-Tropomyosin, Troponin-I Slow-Twitch, Troponin-I Fast-Twitch and Troponin-C Fast'. Biochemical and Biophysical Research Communications, 230, 347-350 (1997).
Muraro T., Stephan D., Tiso N., Zimbello R., Danieli G.A., Hoffman E.H., Valle G. and Lanfranchi G.: 'Chromosomal assignment of 115 expressed sequence tags (ESTs) from human skeletal muscle'. Cytogenet. Cell Genet. 76 (3-4), 144-152 (1997).
Tiso N., Pallavicini A., Muraro T., Zimbello R., Apolloni E., Valle G., Lanfranchi G. and Danieli G.A.: 'Chromosomal Localization of the Human Genes CPP32, Mch2, Mch3 and Ich-1, involved in Cellular Apoptosis'. Biochemical and Biophysical Research Communications 225, 983-989 (1996).
Albertin G., Rossi G.P., Majone F., Tiso N., Mattara A., G.A. Danieli, Pessina A. and Palu' G.: 'Fine Mapping of the Human Endothelin-Converting Enzyme by Fluorescent in Situ Hybridization and Radiation Hybrids'. Biochemical and Biophysical Research Communications 221: 682-687 (1996).
Rampazzo A., Nava a., Eberhard P., Vian E., Slomp P., Tiso N., Thiene G. and Danieli G.A.: 'A new locus for arrhythmogenic right ventricular cardiomyopathy (ARVD2 ) maps to chromosome 1q42-q43'. Human Molecular Genetics vol. 4: 2151-2154 (1995).