Morpholino

MO2-cobl

ID

ZDB-MRPHLNO-110712-2

Name

MO2-cobl

Previous Names

cobl-MOb (1)

Target

cobl (1)

Sequence

5' - CAAGGCTTATCTAGAGATGGAGACA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Splice-blocking MO designed to straddle the intron 7–exon 8 boundary of cobl. cobl-MOb had little effect on Cobl protein levels as detected by immunohistochemistry and caused only modest reduction of wild-type cobl message. ( Ravanelli et al. ZDB-PUB-101209-18 )

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO2-cobl

Expressed Gene	Anatomy	Figures
cobl	whole organism	Fig. 3 from Ravanelli et al., 2011

Phenotype

Phenotype resulting from MO2-cobl

Phenotype	Fish	Figures
cilium assembly process quality, abnormal	EKW + MO2-cobl	Fig. 5 from Ravanelli et al., 2011
Kupffer's vesicle motile cilium decreased length, abnormal	EKW + MO2-cobl	Fig. 5 from Ravanelli et al., 2011

Phenotype of all Fish created by or utilizing MO2-cobl

Phenotype	Fish	Conditions	Figures
cilium assembly process quality, abnormal	EKW + MO1-cobl + MO2-cobl	standard conditions	Fig. 5 from Ravanelli et al., 2011
Kupffer's vesicle motile cilium decreased length, abnormal	EKW + MO1-cobl + MO2-cobl	standard conditions	Fig. 5 from Ravanelli et al., 2011
cilium assembly process quality, abnormal	EKW + MO2-cobl	standard conditions	Fig. 5 from Ravanelli et al., 2011
Kupffer's vesicle motile cilium decreased length, abnormal	EKW + MO2-cobl	standard conditions	Fig. 5 from Ravanelli et al., 2011
heart jogging process quality, abnormal	f1Tg + MO1-cobl + MO2-cobl	standard conditions	Fig. 3 from Ravanelli et al., 2011
heart tube bilateral symmetry, abnormal	f1Tg + MO1-cobl + MO2-cobl	standard conditions	Fig. 3 from Ravanelli et al., 2011

Citations