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Fig. 2 Hepatocyte Vdr impairment stimulates fatty acid storage and diminishes liver growth (A) In situ hybridization for vdra and vdrb in 96-hpf larvae. A dashed line marks the liver. Scale bar, 200 μm. (B) Representative images of cyp24a1mGFP expression at 96 hpf. Dashed lines mark the liver. Scale bar, 100 μm. The number of fish resembling representative images is noted. (C) Analysis of hepatic steatosis and liver size in fabp10a:CFP-NTR larvae following Nile red staining at 7 dpf. Scale bar, 30 μm. (D) Liver lipid droplets per liver volume. Two-tailed Mann-Whitney test. (E) Mean lipid droplet volume. Unpaired two-tailed t test. (F) Left liver lobe volume. Two-tailed Mann-Whitney test. (D–F) n = 15 wild-type (WT), 14 dn-vdra. (G) Hepatocyte quantification in fabp10a:NLS-mCherry larvae at 7 dpf. Scale bar, 30 μm. (H) Left liver lobe hepatocytes. Unpaired two-tailed t test. n = 21 WT, 15 dn-vdra. (I) Analysis of liver lipid content following fasting or 20-h feeding of 0.25% egg yolk and Nile red staining at 7 dpf. The liver is outlined. Scale bar, 30 μm. (J) Liver lipid droplets per liver volume. Two-way ANOVA with Sidak’s multiple comparison test. n = 12 WT.Fast, 12 WT.Yolk, 13 dn-vdra.Fast, 18 dn-vdra.Yolk. (K) Schematic of the BODIPY FL C12 metabolic tracing approach. (L) Maximum intensity projection of livers after 20-h incubation in 0.25% egg yolk solution or fasting followed by feeding of BODIPY FL C12. Scale bar, 10 μm (M) BODIPY+ lipid droplets per liver area. Two-way ANOVA with Tukey’s multiple comparison test. n = 27 WT.Fast, 23 dn-vdra.Fast, 13 WT.Egg, 14 dn-vdra.Egg. Error bars are mean + SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. See also Figure S3 .