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Fig. 5 Time kinetics and dose–response relationship of Stm hit compounds in Stm-infected HeLa cells. (a) HeLa cells were infected with bioluminescent Stm-lux, treated with Stm hit compounds or DMSO at different concentrations and followed over time by measuring emitted light, expressed as relative light units (RLU). DMSO was used at equal % (v/v). Gompertz growth curves were fitted using non-linear regression. (b) Bioluminescence, shown in blue, was measured for HeLa cells infected with Stm-lux after 18 h of treatment with Stm hit compounds at indicated concentrations. RLUs were normalized to the untreated control. Four-parameter logistic regression was used to determine the dose–response relationship between compounds and inhibition of Stm growth, and to determine the IC50 values of compounds. In addition, the cell supernatant was used to determine host cell viability, shown in grey, using LDH-release assays. Again, four-parameter logistic regression was performed to determine the LD50 values of compounds. The data comprises four independent technical replicates.