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Fig. 5 The brain barrier is maintained in the ablated zebrafish (A) Representative images showing how fluorescent intensities of blood plasma, brain parenchyma, and ventricle were measured in the rhombencephalon in control (A1) and ablated fish (A2). (B) Fluorescent intensities in the ventricles in control (DMSO, CtrlMTZ) and ablated fish (MTZ). Statistical analysis: n = 10 except for D/MV ablated group, where n = 4 due to the collapse of the ventricle. Kruskal-Wallis test, p > 0.05. (C) Diagrams showing the location of (A)–(F). (D) Cldn5 immunostainings of TC, fChP, and hChP in control (D1, D2, D4, and D5) and ablated fish (D3 and D6). Max projections. n > 4. (E and F) Transmission electron microscope images of the fChP in control (E) and ablated fish (F). (E1 and F1) Low- (E2–E4 and F2–F6) and high-magnification (E2, F2, and F6) images of junctions, microvilli (E3 and F3), basal membranes (E5 and F5), and motile cilia (E4 and F4). TV, telencephalic ventricle; D/MV, dien-/mesencephalic ventricle; RV, rhombencephalic ventricle; A, anterior; P, posterior; D, dorsal; V, ventral; R, right; L, left. Scale bars: (D) 50 μm, (E1, E3, and F1) 1 μm, and (E2–E5 and F2–F6) 200 nm. See also Figures S11 and S12.