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Fig. 4 Fabp7b-expressing cells are required for the maintenance of the ventricular volume (A) Experimental scheme used for measuring ventricular size and blood-CSF barrier integrity after ablation. (B) Diagram of the juvenile brain with insets indicating the location of (C1)–(C6). (C) Max projection images of the TC, fChP, and hChP in control (C1, C2, C4, and C5) and ablated fish (C3 and C6). (D) Quantification of the TC, fChP, and hChP areas in control (DMSO and CtrlMTZ) and ablated fish (MTZ). n =10. The TC and ChP sizes are shown as normalized GFP+ ratio (mean ± standard deviation). p values from post hoc analysis: TC area: DMSO vs. MTZ, ∗∗p = 0.0055; CtrlMTZ vs. MTZ, ∗∗∗p = 0.0005. fChP area: DMSO vs. MTZ, ∗∗∗p = 0.0002; CtrlMTZ vs. MTZ, ∗∗∗p = 0.0007. hChP area: DMSO vs. MTZ, ∗∗p = 0.0012; CtrlMTZ vs. MTZ, ∗∗∗p = 0.0001. (E) Diagrams of the juvenile brain (top) and ventricles (bottom). Projections of 6 horizontal slices in (F1)–(F3), 5 sagittal slices in (F4)–(F6), and 5 sagittal slices in (F7)–(F9). (F) Horizontal (F1–F3) and sagittal (F4–F6 and F7–F9) views of the ventricles following 10 kDa Alexa 647 injection in control (F1, F2, F4, F5, F7, and F8) and ablated fish (F3, F6, and F9). Ventricles are highlighted with dotted yellow lines. (G) Quantification of the anterior (ant.) TV, posterior (post.) TV, and ant. D/MV and RV areas in the control (DMSO and CtrlMTZ) and ablated fish (MTZ). n = 10. p values from post hoc analysis: ant. TV area: DMSO vs. MTZ, ∗∗p = 0.0077; CtrlMTZ vs. MTZ, ∗∗∗p = 0.0008. post. TV and ant. D/MV areas: DMSO vs. MTZ, ∗∗∗p = 0.0009; CtrlMTZ vs. MTZ, ∗∗∗p = 0.0003. RV area: DMSO vs. MTZ, ∗p = 0.0124; CtrlMTZ vs. MTZ, ∗∗p = 0.0015. (H) Diagrams of the brains in the control and ablated fish. Kruskal-Wallis test. The p values on the graph are the results from Kruskal-Wallis test. ∗∗p < 0.01, ∗∗∗p < 0.001. TV, telencephalic ventricle; D/MV, dien-/mesencephalic ventricle; RV, rhombencephalic ventricle. A, anterior; P, posterior; D, dorsal; V, ventral; R, right; L, left. Scale bars: 50 μm. See also Figures S8–S10.