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Fig. 6 Exogenous melatonin decreases daytime wakefulness. A. Distance traveled (mean and SEM) captured across three continuous days. Melatonin (150 µM final concentration in 0.3% DMSO) or 0.3% DMSO (control) was administered at 11:45 am (right arrow). Activity is captured on a 13:11 h light/dark cycle and shown as zeitgeber time. B. Total distance traveled each hour of light cycle following drug administration. C. Total distance traveled in the hour following drug administration (12 pm–1 pm). Two-way RM-ANOVA with Bonferroni’s multiple comparisons test was performed in B–C. *p < 0.05 adjusted. Data are presented as mean and SEM. n = 8 fish/group from three biological replicates.