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Figure Caption
Fig. 4 Characterization of a ctgfa knock-out zebarfish PAC2 cell line. A Sequencing of ctgfa gene from the single clone screening cells transfected with ZFU6-LentiCRISPRV2-ctgfa. B The mutation type of ctgfa gene from the single clone screening cells. Dash represents the deleted base, red letters indicate the CRISPR target site, green letters indicate the PAM sites. C RT-PCR products of ctgfa. M: DL500 DNA marker
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