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Fig. 2 Simplified diagram of the ZFU6-LentiCRISPRV2 vector adapted from mammal LentiCRISPRV2 vector for gene editing in zebrafish cell. A Diagram of mammal LentiCRISPRV2 vector. B Diagram of ZFU6-LentiCRISPRV2 vector. Colors are represented the names of the mainly regulatory elements including RNA polymerase III promoter (HU6/ZFU6), short EF1alpha (EF1α) promoter, single guide RNA (sgRNA), sgRNA scaffold (scaffold), Cas9 protein (Cas9), and the antibiotic resistance cassette (puromycin)
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