Figure 3

Figure 3

VD regulates glucose metabolism in zebrafish under short-term fasting conditions. (A–C) After the feeding trial, zebrafish fed with VD₃ or non-VD₃ diet were fasted for 24 h before sampling. The gene expression of insra, insrb, gcgra, gcgrb (A) and gck, pklr, pck1, fbp1a (B) in the liver was measured (n = 4/group). Serum glucose levels were determined (C) (n = 3~4 replicates, 4~5 fish/replicate). (D–F) WT and cyp2r1^−/− zebrafish at 3 mpf were fasted for 24 h before sampling. The gene expression of insra, insrb, gcgra, gcgrb (D) and gck, pck1 (E) in the liver was analyzed (n = 4/genotype). Serum glucose levels were determined (F) (n = 4 replicates, 3 fish/replicate). * p < 0.05, ** p < 0.01, *** p < 0.001, ns: no statistical significance.