Fig. 6

Fig. 6 Analysis of locomotor behavior in calretinin knock-down larvae during swims and escapes. A) Parameters indicating swimming performance, (total distance, maximum acceleration, and mean velocity, at 4, 6 and 10 days post fertilization (dpf) during the free swimming in controls and calretinin knock-down larvae. B) Distance moved, maximum acceleration and maximum velocity during escape responses at 4, 6 and 10 dpf in freely moving control and calretinin knock-down larvae. C) Probability density distributions of fictive swim frequency in spontaneous and optomotor response evoked swims in 5–6 dpf calretinin knock-down larvae (N = 9, n = 1323) and control larvae (N = 6, n = 788). D) Example of a regular swim of a control larvae. E) Example of simultaneous contralateral bursting in a calretinin knock-down larvae. F) Example of inconsistent burst duration and amplitude in a calretinin knock-down larvae. G) Example of a generally incoherent swim in a calretinin knock-down larvae. H) Representative example of a stereotyped C-start escape swim in a control larvae consisting of a 100ms fast phase (>30Hz) followed by a slow phase (<30Hz). I) Calretinin knock-down larvae (N = 7, n = 135) showed a lack of fast swim speeds during the initial phase of a C-start escape compared to control larvae (N = 4, n = 86) (P < 0.005). J) Duration of C-start escape swims is shorter for calretinin knock-down larvae (P < 0.01). K) Example of qualitatively altered escape swim in calretinin knock-down larvae consisting of a short period of strongly lateralized activity. L-M) Examples of calretinin knock-down larvae escapes that show no (L) or reduced (M) activity during the supposed fast phase of the escape swim. Statistical differences: >0.05 (ns), ≤0.05 (*), ≤0.01 (**), ≤0.001 (***) and ≤0.0001 (****).