Fig. 4

Fig. 4 Calretinin deficient larvae displayed a reduced amount of dmrt3aRFP cells. A) Brightfield images of calb2a, calb2b double morphants versus controls at 3 days post fertilization (dpf). B) Confocal images of immunohistochemistry against calretinin in controls and calretinin knock-down larvae. C) Confocal images showing dmrt3aRFP transgenic line in control and calretinin knock-down larvae at 3 dpf. D) Confocal images showing dmrt3aRFP transgenic line in control and calretinin knock-down larvae at 6 dpf. Arrowheads indicate CoLo axons. E) Quantification of dmrt3aRFP at 3 dpf. F) Quantification of dmrt3aRFP at 6 dpf. Scale bar, 50 μm. Statistical differences: >0.05 (ns), ≤0.05 (*), ≤0.01 (**), ≤0.001 (***) and ≤0.0001 (****).