Fig. 1 Chemical structure of PALM (A) and PALM effect in the EKP-induced hyperlocomotion assay in larval zebrafish. Zebrafish larvae (6 dpf) were incubated with PALM (75, 150, 300 or 450 µM) for 24 h and then exposed to EKP (400 µM). The analysis started 5 min after EKP application. Results of the experiments are shown as: (B) total distance traveled during 30 min of experiment, (C) distance traveled in 2 min long time bins, (D) number of events [nr/20 min] and (e) mean duration of events [s/20 min] in the LFP recordings. Two-way ANOVA with repeated measures with Bonferroni’s post-hoc test (A) and one-way ANOVA with Tukey’s post hoc test (B) were applied for statistical purposes. Data are shown as mean ± SEM. Behavioural studies (B, C): vehicle + vehicle, n = 33; PALM 450 + vehicle, n = 28; vehicle + EKP, n = 91; PALM 75 + EKP, n = 48; PALM 150 + EKP, n = 48; PALM 300 + EKP, n = 37; PALM 450 + EK; n = 87; LFP recordings (D, E): vehicle + vehicle, n = 11; PALM 450 + vehicle, n = 5; vehicle + EKP, n = 18; PALM 450 + EKP 400, n = 15. * p < 0.05, * * p < 0.01, * ** P < 0.001. EKP – ethyl 2-ketopent-4-enoate, PALM – palmatine.
Image
Figure Caption
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Biomed. Pharmacother.