ZFIN Image: Zhou et al., 2024, Figure 4

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Figure Caption

Figure 4

The effects of lncRNA gas5 knockout. (a) The expression level of lncRNA gas5 knockout embryos, detected by qPCR. Data derived from three independent experiments are shown as mean ± SEM. **** indicates p < 0.0001. (b) The expression level of lncRNA gas5 knockout embryos, detected by in situ hybridization. NI represents no injection, control represents only injection of Cas9 protein, Cas9 represents co-injection of sgRNA and Cas9 protein. Microscope’s magnification: 45 × (c–h) The effects of knockout on embryonic development. Microscope’s magnification: 45 ×. #: enlargement of yolk extension. *: small or absent eyes, arrows indicate nonseparation of two otoliths.

Acknowledgments

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