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Fig. 6 Top chart shows melatonin levels secreted from pineal glands superperfused with a constant flow rate of 5 µL/min with saline or melatonin. Fractions were collected at 30 min intervals. Each point represents the mean ± SEM (saline and 1 mM n = 5, 5 and 50 nM n = 6). Grey bar indicates superfusion period. Experiments were performed in triplicate. Bottom chart displays average levels of melatonin release from pineal glands after superfusion. Each bar represents the mean ± SEM (n = 10) from all collected fractions (saline and 1 mM n = 45, 5 and 50 nM n = 54).
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Full text @ J. Pineal Res.