|
Fig. 2 Zebrafish transformed by mutant hRAB40B showed motility defects. (A) Developmental morphologies of control, Mock or exogenous hRAB40B-Y83X mRNA-expressed larvae at 84 hpf. Scale bars, 2,000 μm. (B) Heatmap images showing swimming patterns between the control and hRAB40B-Y83X mRNA-injected zebrafish larvae. (C) Quantification of velocity analyzed in control zebrafish (n=65), Mock-expressing zebrafish (n=70), and hRAB40B-Y83X-transformant zebrafish (n=76). (D) Lateral view images after staining with α-SV2 (presynaptic region) and α-BTX (postsynaptic region) of the whole-mounted zebrafish at 84 hpf. Scale bars, 50 μm. (E) Comparison of NMJ signal ratios within ROI among control (n=43), Mock-expressing zebrafish (n=20), and hRAB40B-Y83X-transformant zebrafish (n=22). Comparisons of NMJ innervation in the presynaptic area (F) and postsynaptic area (G) among control (n=40), Mock-expressing zebrafish (n=23) and hRAB40B-Y83X-transformant zebrafish (n=20). Statistical significance was determined using an unpaired Student’s t-test (**p<0.01, ***p<0.001).