Fig. 5.

Fig. 5.

Unsupervised clustering of nuclei in shape space. (A) UMAP of neuromast cells, color coded by nucleus shape cluster. Colors and associated cluster numbers are used in B-F. (B) PAGA plot of nucleus shape clusters. Each node represents a cluster. The thickness of the line drawn between nodes indicates the estimated connectivity between clusters (the value of which is shown adjacent to the corresponding line). Edges with connectivity below 0.1 are not depicted. (C) 3D projections of representative cells for each nuclear cluster, defined as the cell closest to the cluster centroid in four-dimensional PC space. The original segmentation masks are depicted (not SHE reconstructions). (D) Polar plots of relative locations of cells within each cluster. (E) Distributions of the cell distance from the neuromast center for each cluster. Dashed lines indicate quartiles. (F) Distributions of neuromast cell angles for each cluster. Dashed lines indicate quartiles. Angles were calculated with reference to the positive x-axis extending from the neuromast centroid.