Fig. 4.

Fig. 4.

Unsupervised clustering of cells in shape space. (A) UMAP of neuromast cells, color coded by cell shape cluster. Colors and associated cluster numbers are used in B-F. (B) PAGA plot of cell shape clusters. Each node represents a cluster. Thickness of the line drawn between nodes indicates estimated connectivity between clusters (the value of which is shown adjacent to the corresponding line). Edges with connectivity below 0.1 are not depicted. (C) 3D projections of representative cells for each cluster, defined as the cell closest to the cluster centroid in eight-dimensional PC space. (D) Polar plots of relative neuromast locations of cells within each cluster. (E) Distributions of the cell distance from the neuromast center for each cluster. Dashed lines indicate quartiles. (F) Distributions of neuromast cell angles for each cluster. Dashed lines indicate quartiles. Angles were calculated with reference to the positive x-axis extending from the neuromast centroid. Cluster 1 shows a bias towards the dorsoventral poles (90° and −90°), whereas cluster 2 shows a bias to the anterior-posterior compartments (0°, 180° and −180°).