Fig. 2

Fig. 2 neurog1 and olig2 single and double mutant phenotypes and Notch inhibition reveal potential epistatic relationships. (A–H) th expression in (A,E) wildtype, (B,F) neurog1hi1059/hi1059 mutants, (C,G) olig2m1317/m1317 mutants and (D,H) neurog1hi1059/hi1059; olig2m1317/m1317 double mutants in (E–H) DAPT treated embryos compared to (A–D) control siblings at 72 hpf. The numbers 1-6 mark the corresponding (DAC1-DAC6) dopaminergic cell clusters (A). The number of th+ DAC5 and DAC6 cells is increased in WT embryos (E) after DAPT treatment, compared to (A) control embryos (filled black arrowheads). The number of th+ DAC6 cells in olig2m1317/m1317 mutant embryos (G) after DAPT treatment is increased compared to control olig2m1317/m1317 mutant embryos (C, open arrowheads). DAPT treatment does not alter the number of th+ cells in neurog1hi1059/hi1059 or double mutant embryos (B,F,D,H). (I) Quantification of th+ cells in single and double mutants compared to wildtype siblings at 72 hpf. The number of embryos analyzed for each genotype are indicated (n-numbers in grey box); p-values were calculated using the Mann-Whitney test. p-values indicate significance if they are <0.05 (*, 0.01–0.05; **, 0.001–0.01; ***, 0.0001–0.001; ****, <0.0001). For numbers see Supplemental Table 2. Abbreviations: numbers 1 through 6 for DAC1-6, dopaminergic cell cluster 1–6; LC, locus coeruleus; PO, preoptic area. All images show single optical planes of dorsal views. Anterior is to the left. Scale bars: 100 μm.