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Fig. 3 Behavioural alterations observed in the rbfox1sa15940 line.A Open field test. Time spent in the centre of the arena, time spent freezing and total distance travelled during the open field test. One-way ANOVA followed by Tukey’s multiple comparison test. B Visually-mediated social preference test (VMSP). Social preference step. Time spent in the area close to the 1st strangers and in the opposite area, time spent freezing and total distance travelled during the social preference step of the VMSP test. Two-way ANOVA followed by Sidak’s multiple comparison test. C Visually-mediated social preference test (VSMP). Preference for social novelty step. Time spent in the areas close to the 1st or 2nd strangers, time spent freezing and total distance travelled during the preference for social novelty step of the VMSP test. Two-way ANOVA followed by Sidak’s multiple comparison test. D Shoaling test. Mean of interindividual distance, nearest neighbour distance, cluster score and total distance travelled during the shoaling test. One-way ANOVA followed by Tukey’s multiple comparison test and Kruskal-Wallis followed by Dunn’s multiple comparisons test. E Black and white test. Number of crossings between areas and time spent in the white area of the tank during the black and white test. Kruskal-Wallis followed by Dunn’s multiple comparisons test. F Mirror test. Time spent exhibiting an aggressive behaviour against the mirror. For all the experiments except for the shoaling test: HOM, rbfox1sa15940/sa15940 fish; HZ, rbfox1sa15940/+ fish; WT, wild-type TL. n = 13 WT, 13 HZ and 13 HOM for all tests except for the shoaling test. For the shoaling test: n = 2 groups of 5 individuals per genotype. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Mean ± SD.